天胡荽属药用植物的积雪草苷与羟基积雪草苷的含量测定  

Determination of Asiaticoside and Madecassoside in Hydrocotyle

作　　者：董关涛[1] 宋良科[1] 汤昊[1] 王岩[1] 王小宁[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中国野生植物资源》2012年第2期47-50,共4页Chinese Wild Plant Resources

基　　金：西南交通大学基金(2006B10);国家自然保护区生物标本-民族医药标本整理整合(2005DKA21404)

摘　　要：目的:建立天胡荽属植物的积雪草苷和羟基积雪草苷HPLC含量分析的方法,为开发利用天胡荽属植物药用资源奠定基础。方法:以积雪草苷和羟基积雪草苷为指标成分,采用高效液相色谱法,以Symmetry C18(4.6 mm×250 mm,5μm)为色谱柱;乙腈-水(29∶71,v/v)为流动相;流速0.8 mL/min;柱温30℃;检测波长210 nm,外标法进行测定。结果:积雪草苷的回归方程为:Y=34 958X+154 822 R2=0.999 1(n=8),羟基积雪草苷的回归方程为:Y=114 318X-21 002 R2=0.999 2(n=8),结果表明线性关系与分离度良好,平均加样回收率为99.98%、100.53%,RSD为1.86%、2.31%。结论:该方法稳定性、重复性良好,可做为天胡荽属药用资源的评价方法之一,实验结果为野生与栽培资源的开发与利用奠定了基础。Objective： To establish a stable method for the content determination of asiaticoside and madecassoside in Hydrocotyle.And lay a foundation for the further study.Method： The HPLC was performed using Symmetry C18 column（4.6 mm×250 mm,5 μm） and acetonitrile-water（29∶71） as the mobile phase.The flow rate was 0.8mL/min,the detection wavelength was 210 nm,and the column temperature was 30 ℃.The external standard method was used as quantitative analysis.Results： the regression equation of asiaticoside was Y=34 958X＋154 822 R2=0.999 1（n=8）,the regression equation of madecassoside was Y=114 318X-21 002 R2=0.999 2（n=8）;the average recovery was 99.98%、100.53%,RSD was 1.86%、2.31%.Conclusion： The method is stable and reliable.It can be used for quality control of Hydrocotyle.And the result provides scientific basis for exploiting wild and plant resources.

关 键 词：天胡荽属植物 积雪草苷 羟基积雪草苷 HPLC 

分 类 号：R284[医药卫生—中药学]