油茶芽苗砧嫁接体愈合过程AFLP分析  

AFLP analysis of nurse seed grafted union in Camellia oleifera during healing

作　　者：冯金玲[1] 杨志坚[1] 陈辉[1] 

机构地区：[1]福建农林大学,福建福州350002

出　　处：《中南林业科技大学学报》2012年第3期141-146,共6页Journal of Central South University of Forestry & Technology

基　　金：科技人员服务企业行动项目(2009GJC40006);福建省科技平台建设项目(2010N2001)

摘　　要：为了探讨油茶芽苗砧嫁接体愈合过程中嫁接口和接穗是否发生基因变化,利用AFLP对不同发育阶段的油茶芽苗砧嫁接口,油茶芽苗砧愈伤口以及接穗母树、砧木和接穗萌发芽的基因进行分析。结果表明:油茶芽苗砧嫁接口发育过程中,条带数变化相对比较大,特别是在嫁接移栽后的第4、16、24、35天,愈伤口条带数的变化是在移栽后第0天至第18天间;接穗母树、接穗萌发芽和砧木存在13条特异带,10条在接穗萌发芽DNA中存在,其中5条为接穗萌发芽特有条带,5条在砧木茎中存在。To investigate whether gene changes of the nurse seed grafting union and the scion during healing of the nurse seed grafted union in Camellia oleifera.The differences of development between the nurse seed grafting unions in Camellia oleifera and the healing wound,the mother tree of the scion,the root stock and the buds from the scion were examined by AFLP（Amplified Fragment Length Polymorphism） technology.The results show that the number of bands changed relatively during the development of the nurse seed grafting union,especially at the days 4th,16th,29th and 35th after grafting;the change in number of bands during the development of the healing wound took place at the days 0～18th after transplantation.There were 13 specific bands in the gene among the scion,the root stock and the buds from the scion.Of them,10 bands belonged to the buds from the scion DNA,5 bands of which only belonged to it,the rest bands were found on both it and the root stock.The band change of the nurse seed grafting may caused by DNA methylation.The changes of the scion may resulted from DNA methylation,or affected by the pressure of the graft,may gene exchanged between the scion and the root stock.The real causes of the changes will be studied furtherly.

关 键 词：油茶 芽苗砧嫁接 基因 AFLP 

分 类 号：S794.4[农业科学—林木遗传育种]