Notchl在人乳腺癌细胞中的表达及其与乳腺癌耐药的关系  

Relationship between expressions of Notchl and drug resistance of breast carcinoma

作　　者：逯种[1] 刘春萍[1] 郭辉[1] 田元[1] 张景辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院乳腺甲状腺外科,武汉430022

出　　处：《中华实验外科杂志》2012年第5期818-820,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30972935）

摘　　要：目的检测乳腺癌细胞株McF-7与MCF-7／ADR的Notchl表达、成球能力、干细胞池比例及干细胞内Notchl的表达，探讨Notchl在逆转乳腺癌化疗耐药中的意义。方法实时荧光定量逆转录一聚合酶链反应（RT—qPCa）及Westernblot法检测乳腺癌细胞株MCF-7、MCF-7／ADR及表型为ALDH1＋／CD44＋／CD24-乳腺癌干细胞中Notchl的表达；无血清培养检测MCF-7及MCF-7／ADR的成球能力；流式细胞仪检测MCF-7与MCF-7／ADR的干细胞比例并分选干细胞。结果MCF-7／ADR的Notch1的表达明显高于MCF-7（0．0748±0．0043比0．0461±0．0022，P〈0．05），微球体形成能力也明显高于MCF-7（P〈0．05）；流式细胞仪检测干细胞比例，MCF-7／ADR高于MCF-7（11．40％比1．89％）；分选出的干细胞Notchl的表达明显高于非干细胞（0．3096±0．0324比0．0428±0．0061，P〈0．05）。结论Notchl在乳腺癌及乳腺癌干细胞中高表达，干预Notchl可能可以逆转乳腺癌治疗的耐药。Objective To investigate the role of Notchl in the chemotherapy process of breast cancer by studying the the efficiency of mammosphere formation, the ratio of stem cells in MCF-7 and MCF-7/ADR, and the expression status of Notchl in MCF-7, MCF-7/ADR and stem cells respectively. Methods Real time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-qPCR） and Western blotting were employed to detect the expression status of Notchl in breast cancer cell lines MCF-7, MCF-7/ADR and ALDH1 ＋/CD44 ＋/CD24- breast cancer stem cells （BCSCs）. Serum-free me- dium was available to assess the efficiency of mammosphere formation. Flow cytometry was used to define the ratio of BCSCs and select them in MCF-7 and MCF-7/ADR. Results The expression status of Notchl [ （0. 0748±0. 0043 ） vs （0. 0461±0. 0022 ）, P 〈 0.05 ] and the ability of mammosphere formation were significantly higher in MCF-7/ADR than in MCF-7 （P 〈 0. 05）. There was significant difference in the ra- tio of BCSCs between MCF-7/ADR and MCF-7 （ 11.40% vs 1.89% ）. Furthermore the expression score of Notchl in BCSCs was significantly higher than in non-BCSCs [ （0. 3096±0. 0324 ） vs （0. 0428 ±0. 0061 ）, P 〈 0.05 ]. Conclusion The high level expression of Notchl in breast cancer and BCSCs suggests regulating Notchl may play an important role in reversing chemotherapy resistance of breast cancer.

关 键 词：乳腺肿瘤 干细胞 NOTCHL 

分 类 号：R737.9[医药卫生—肿瘤]