人血管内皮生长因子165对肝硬化大鼠肝部分切除术后余肝再生能力的影响  

Effects of human vascular endothelial growth factor 165 on liver regeneration in hepatocirrhosis after partial hepatectomy in mice

作　　者：李海军[1] 易超[1] 王喜艳[1] 

机构地区：[1]新疆医科大学附属肿瘤医院肝胆胰外科,乌鲁木齐830011

出　　处：《中华实验外科杂志》2012年第5期865-868,共4页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区自然科学基金资助项目（2010211A28）

摘　　要：目的观察外源性人血管内皮生长因子165（VEGF165）对肝硬化大鼠肝部分切除术后余肝再生及肝功能恢复的影响。方法采用基因重组技术构建人VEGFl65慢病毒表达载体，传统CCl4诱导法制备大鼠肝硬化模型；126只肝硬化大鼠行50％肝切除术后随机分为3组：A组（VEGF165组）、B组（空病毒组）、c组（对照组），分别于术前、术后12、24、72h、7、14、28d检测各组大鼠余肝组织VEGF165mRNA与蛋白的表达、肝再生率、细胞核抗原（PCNA）阳性表达率及门静脉血液中丙氨酸转氨酶（ALT）与天冬氨酸转氨酶（AST）含量。结果人VEGF165慢病毒表达载体成功构建且可使大鼠肝脏表达VEGF165mRNA及蛋白；肝硬化大鼠模型制模成型率为96．18％；VEGF165组术后72h至术后28d肝再生率分别为（34．98±6．22）％、（62．19±1．81）％、（83．54±4．50）％、（92．23±4．41）％，术后24h至术后28dPCNA阳性表达率分别为（18．05±0．92）％、（42．89±5．52）％、（35．56±3．86）％、（26．37±1．35）％、（19．48±2．70）％均显著高于对照组（P〈0．01）；术后72h至术后28d血清ALT含量分别为（258．14±12．94）、（215．67±22．12）、（175．92±8．78）、（133．26±13．92）U／L，血清AST含量分别为（553．34±14．10）、（481．37±14．74）、（425．95±5．04）、（388．29±15．99）U／L均较对照组明显下降（P〈0．01）。结论外源性人VEGF165可促进肝硬化大鼠肝部分切除术后余肝的再生及肝功能的恢复。Objective To study the effects of exogenous vascular endothelial growth factor 165 （VEGF165） on liver regeneration and the recovery of liver function in hepatic cirrhosis after partial hepa-tectomy in rats. Methods The lentivirus vector containing human VEGF165 gene was constructed by using gene recombinant technology. CC14-induced cirrhotic models in 150 rats were established. The 126 cirrhot- ic rats after hemihepatectomy were randomly divided into three groups： group A （VEGF165 group）, group B （empty virus group） and group C （control group）. The expression of VEGF165 mRNA and protein in liver tissue, liver regeneration rate, the positive rate of proliferating cell nuclear antigen （PCNA）, alanine transaminase （ALT） and aspartate aminotransferase （AST） contents in the blood of portal vein were detec- ted before and 12 h, 24 h, 72 h, 7 days, 14 day, and 28 days after operation. Results The lentivirus vector construction was proved to be correct and could make the rats liver expressing VEGF165 mRNA and protein stably. The success rate of cirrhotic models in rats was 96. 18%. The liver regeneration rate （34.98±6.22）%, （62.19±1.81）%, （83.54±4.50）% and （92.23±4.41）% during 72 h to 28 days after operation and the positive rate of PCNA （18. 05±0. 92）%, （42. 89±5.52）%, （35.56±3.86）%, （26. 37±1.35）% and （19.48±2. 70）% during 24 h to 28 days after operation in VEGF165 group were obviously higher than those in the control group （P 〈 0. 01 ）. The serum concentrations of ALT （258. 14±12. 94）, （215.67±22.12）, （175.92±8.78） and （133.26±13.92） U/L and AST （553.34±14.10）, （481.37±14.74）, （425.95±5.04）, （388.29±15.99） U/L during 72 h to 28 days after operation in VEGF165 group were evidently lower than those in the control group （P 〈 0.01 ）. Conclusion The exogenous VEGF165 has significantly positive effect on liver regeneration and the recovery of liver function after partial hepateetomy in rats with hep

关 键 词：肝硬化 肝部分切除术 肝再生 血管内皮生长因子165 慢病毒载体 

分 类 号：R657.3[医药卫生—外科学]