肝移植术后潜在免疫耐受者外周血自然杀伤细胞、调节性T细胞和记忆性T细胞的表达及其临床意义  

Increased peripheral NK cells, regulatory T cells and memory T cells profiling in the potential toler- ance liver recipients

作　　者：邓斐文[1] 朱晓峰[1] 马毅[1] 何晓顺[1] 郭志勇[1] 刘龙山[1] 李强[1] 

机构地区：[1]中山大学附属第一医院器官移植科,广州510080

出　　处：《中华实验外科杂志》2012年第5期949-952,共4页Chinese Journal of Experimental Surgery

基　　金：国家“十一五”科技支撑计划资助项目（20008BA16B06）;广东省科技计划资助项目（20108031600222）;卫生部临床重点资助项目（81102244、81102245、81170448）

摘　　要：目的探讨肝移植术后潜在免疫耐受者免疫状态，为临床指导抗排斥治疗提供依据。方法纳入1999年1月至2007年12月在本中心行肝移植存活至今潜在免疫耐受者29例，分为3～5年组（3Y组）10例，5～11年组（5Y组）19例，另纳入肝移植术后反复排斥反应者（RJ）10例，年龄匹配健康对照组（HC）17例；流式检测外周血单个核细胞中自然杀伤（NK）细胞、记忆性T细胞、调节性T细胞（Treg）3个亚群的表达。结果3Y、5Y组CD4＋CD25＋T细胞（3Y：27．56±4．63；5Y：30．07±3．91）、静息性Treg占CD4＋T细胞比例（3Y：0．22±0．05；5Y：0．17±0．03）比RJ组明显升高（CD4’CD25＋T：11．78±4．28；静息性Treg：0．05±0．02）（P〈0．05）；5Y组NK细胞占淋巴细胞比例（29．11±3．31）比RJ组（13．92±3．11）明显升高（P〈0．05）；效应性记忆性CD8’T细胞比例3Y组（12．92±2．05）比RJ组（8．74±1．69）明显升高（P〈0．05）。结论肝移植术后潜在免疫耐受者外周血NK细胞、静息性调节性T细胞和效应性记忆性CD8＋T细胞比例相对排斥反应者明显升高，可作为潜在的耐受标志物。Objective To analyze the immune status of the potential tolerance liver recipients, in order to help guiding the immunosuppressant adjustment in the future. Methods Twenty nine recipients had normal liver function until now with single immunosuppressive drug who underwent liver transplantation from January 1999 to December 2007 in our center were included. They were divided into two groups, po- tential tolerance 3Y group included 10 cases who were 3-5 years （included 5 years） after transplantation, and 5Y group included 19 cases who were more than 5 years after transplantation; Another I0 liver recipi- ents occurred acute rejection twice or more and 17 age matched healthy control individuals were included as RJ and HC group respectively. The natural killer （NK） cells, memory T cells, regulatory T cells and its new three subsets were detected with flow cytometry. Results The percentage of CD4＋ CD25＋ T cells （3Y ： 27. 56 ±±4. 63 ; 5Y ： 30. 07 ±3.91 ） and resting regulatory T cells （3Y ： 0. 22±0.05 ; 5Y： 0. 17±0.03 ） within the CD4 ＋ T cells in the 3Y and 5Y group were significantly higher than that in the RJ group （ 11.78 ±4. 28, 0. 05 ± 0. 02）, P 〈 0. 05. The proportion of NK cells among lymphocytes increased significantly in the 5Y group （29. 11 ＋ 3.31 ） compare with the RJ group （ 13.92±3.11 ） P 〈 0. 05 ; The effector memory CD8 ＋ T cells was significantly higher in the 3Y group （ 12. 92±2. 05 ） compared with the RJ group （8.74 ± 1.69）, P 〈 0.05. Conclusion The proportion of NK ceils, resting regulatory T cells and effector memory CD8＋T cells increased significantly in the potential tolerance liver recipients, these pa- rameters might be the potential tolerance biomarkers.

关 键 词：肝移植 免疫耐受 自然杀伤细胞 调节性T细胞 记忆性T细胞 

分 类 号：R392[医药卫生—免疫学]