大鼠低摄钙相关性高草酸尿症模型的建立  

Establishment of A Rat Model of Low Calcium Diet Related Hyperoxaluria

作　　者：田晶[1] 郭宏骞[2] 孙西钊[2] 孙则禹[2] 鲁德生[1] 

机构地区：[1]南京大学医学院附属鼓楼医院急诊科,南京210008 [2]南京大学医学院附属鼓楼医院泌尿外科,南京210008

出　　处：《中国医学科学院学报》2012年第2期134-137,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的建立大鼠低摄钙相关性高草酸尿症模型并进行初步探讨。方法健康雄性SD大鼠24只,用随机区组设计法分为低剂量钙组、中等剂量钙组及高剂量钙组,连续喂养3 d,各组大鼠在3 d内分别依次给予剂量递减的饮食钙。测量大鼠每天的24 h尿量、尿草酸浓度、尿肌酐浓度并计算24 h尿草酸总排泄量。其中,尿草酸浓度用高效液相色谱法检测,尿肌酐浓度用自动生化分析仪检测。结果 24 h尿草酸总排泄量随时间变化而变化(F=7.893,P<0.01);时间因素与分组因素之间无交互作用(F=1.142,P>0.05);24 h尿草酸总排泄量随分组变化而变化(F=3.565,P<0.05)。3组大鼠第3天的24 h尿草酸总排泄量均显著高于第1天(P<0.05)。结论通过控制饮食钙摄入水平,成功建立了大鼠低摄钙相关性高草酸尿症模型。Objective To establish a rat model of low calcium diet related hyperoxaluria and explore its features.Methods By means of randomized blocks design,totally 24 SD male rats were divided into low calcium diet group,medium calcium diet group,and high calcium diet group.Each group was sequentially fed on different calcium diets for 3 days.The urinary volume within 24 hours was recorded,the consistency of urinary oxalate by high-efficiency liquid chromatography,and the consistency of urine creatinine by automatic biochemical analyzer.The consistency was corrected to the output of urinary oxalate of rats in 24 hours,and the results were evaluated by repeated measurement of variance analysis and multivariate analysis of variance.Results The output of urinary oxalate of rats in 24 hours varied with time（F=7.893,P〈0.01）.The time factors showed no association with the group division（F=1.142,P〈0.05）.The output of urinary oxalate of rats in 24 hours varied with group division（F=3.565,P〈0.05）.The output of urinary oxalate in 24 hours in three groups on the third day was significantly higher than that on the first day（P〈0.05）.Conclusion By controlling the calcium intake,we successfully established the model of low calcium diet related hyperoxaluria in rat.

关 键 词：草酸钙结石 饮食钙 高草酸尿症 高钙尿症 

分 类 号：R691.4[医药卫生—泌尿科学]