复发性流产患者HLA及血小板特异性糖蛋白抗体GPⅡb/Ⅲa表达的初步研究  

Preliminary study on the expression of HLA and GP Ⅱb/Ⅲa antibodies in recurrent spontaneous abortion pregnant

作　　者：尹爱华[1] 邓晶[2] 吴菁[1] 徐秀章[2] 夏文杰[2] 丁浩强[2] 陈扬凯[2] 叶欣[2] 

机构地区：[1]广东省妇幼保健院产前诊断中心,广州510010 [2]广州血液中心临床输血研究所,广州510095

出　　处：《中国免疫学杂志》2012年第4期338-340,共3页Chinese Journal of Immunology

基　　金：广东省科技攻关计划课题(No.2009B030801255);广州市医药卫生科技重点项目(201202A212001)资助

摘　　要：目的:探讨原因不明复发性流产(RSA)患者HLA抗体和血小板特异性糖蛋白(GPⅡb/Ⅲa)抗体的表达情况。方法:对299名复发性流产的患者血浆中HLA抗体和GPⅡb/Ⅲa抗体进行检测,血小板抗体检测试剂盒(固相凝集法)筛查IgG抗体,Antigen Tray-LATM板检测HLA抗体,GTI公司PAK-PLUS检测试剂盒检测血小板特异性抗体,利用流式细胞仪技术进行GPⅡb/Ⅲa抗体的确证。结果:检测的299例RSA患者中,IgG抗体阳性26例,阳性率8.69%。26例IgG抗体阳性患者中,25例HLA抗体呈阳性。HLA-Ⅰ类抗体阳性占5.02%,HLA-Ⅱ类抗体阳性占1.33%,HLA-Ⅰ+Ⅱ类抗体阳性占2.01%;在26例RSA患者中,GPⅡb/Ⅲa抗体阳性4例(占15.38%),其中GPⅡb/Ⅲa阳性和HLA抗体均阳性3例(占11.54%),GPⅡb/Ⅲa抗体单独阳性1例(占3.85%)。结论:初步探讨了RSA患者HLA抗体和GPⅡb/Ⅲa抗体的分布情况,本研究为复发性流产患者血小板抗体的检测提供了初步的实验基础。Objective： To investigate the expression of HLA antibody and platelet-specific glycoprotein（GP Ⅱb/Ⅲa） antibody in the recurrent spontaneons abortion patient.Methods： 299 recurrent spontaneous abortion patients were studied.The IgG antibodies were detected with Solid-phase Coombs Test Kit for Platelet Antibody Assay.GP Ⅱb/Ⅲa antibodies were detected with GTI PAK-PLUS KIT.The expression of HLA antibodies were determined by Antigen Tray-LATM.Flow cytometry technology for conclusive evidence of GP Ⅱb/Ⅲa antibodies.Results： Total of 299 RSA patients,the IgG antibody in 26 cases were positive（8.69%）,HLA antibody in 25 cases were positive.There are 5.02% cases expression of HLA-Ⅰ antibodies,1.33% cases expression of HLA-Ⅱ antibodies,2.01% cases expression of both HLA-Ⅰ and HLA-Ⅱ antibodies.In the 26 cases,there are 4 cases expression of GP Ⅱb/Ⅲa antibodies（15.38%）,just one case expresses the GP Ⅱb/Ⅲa antibody only.Conclusions： The results show that the expression of HLA antibody and GP Ⅱb/Ⅲa antibody in the RSA patients.Platelet antibodies are important for the RSA patients.

关 键 词：HLA GPⅡb/Ⅲa 复发性流产 

分 类 号：R392.1[医药卫生—免疫学]