可溶性B7-H3(sB7-H3)在急性白血病中的检测及临床意义  

Detection and clinical significance of the soluble B7-H3 in acute leukemia

作　　者：孙海洪[1] 张光波[2] 史进方[3] 李淑湘[2] 顾文超[1] 石小蕊[1] 周迎会[1] 张学光[2] 

机构地区：[1]苏州大学医学部生物化学与分子生物学系,苏州215123 [2]苏州大学医学生物技术研究所江苏省临床免疫重点实验室,苏州215007 [3]苏州大学附属第一人民医院,苏州215006

出　　处：《中国免疫学杂志》2012年第4期362-364,367,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30901789;30930085;30771958);国家重点基础研究项目(2007CB512402);江苏省卫生厅自然科学基金(H200961)的资助

摘　　要：目的:检测sB7-H3在急性白血病病人脑脊液中的不同表达,以及分析该分子的表达与急性白血病的亚型的相关性。方法:采用ELISA的方法来检测sB7-H3在176位白血病病人脑脊液中的表达。依据国际公认的FAB(French-Amer-ican-British)分类系统将急性白血病病人分型。结果:sB7-H3在急性淋系白血病与急性髓系白血病中的表达差异显著(P=0.0381),这与人白血病细胞株的检测结果一致;在急性淋系白血病的亚型中,sB7-H3的表达没有显著性差异;在急性髓系白血病的亚型中,sB7-H3的表达在M3与M5和M4与M5之间存在显著性差异(分别为P=0.024 3和P=0.022 3)。结论:sB7-H3的表达与白血病的分型具有一定的相关性,同时也进一步证实B7-H3参与了人体免疫平衡的调节,可能会为今后急性白血病的临床诊断提供依据。Objective：To detect the different expression of soluble B7-H3（sB7-H3） in acute leukemia and analyze the association between the sB7-H3 level and the classification of acute leukemia.Methods：Enzyme-linked immunosorbent assay kits were used to measure the expression of sB7-H3 in cerebrospinal fluid（CSF） of 176 patients with acute leukemia.Classification of acute leukemia was accordance with the FAB（French-American-British） classification system.Results：There was signicant difference between acute lymphocyte leukemia（ALL） and acute myeloid leukemia（AML,P=0.038 1）,which was the same as what in some human leukemia cell strains.However,there was no statistically signicant difference among the subtypes of ALL,but in the CSF of patients with AML,the level of sB7-H3 was significantly different between M3 and M5（P=0.024 3）,M4 and M5（P=0.022 3）.Conclusion：The level of sB7-H3 is associated with the classification of acute leukemia.B7-H3 has a role in the immune system balance.It may be useful in clinical diagnosis as a new technique.

关 键 词：急性白血病 sB7-H3 亚型 脑脊液 

分 类 号：R392-33[医药卫生—免疫学]