HPLC测定水产品中四环素类抗生素残留量  

Determination of residual tetracycline antibiotics in the aquatic product by HPLC

作　　者：廖先智[1] 张灵丽[1] 胡六江[1] 董华平[1] 

机构地区：[1]绍兴文理学院化学化工学院,浙江绍兴312000

出　　处：《化学工程师》2012年第4期23-24,共2页Chemical Engineer

基　　金：浙江省科技厅科技计划分析测试基金资助项目(2011C37012);浙江省实验教学示范中心实验教学改革项目资助项目;绍兴文理学院学生科研资助项目

摘　　要：本文建立了水产品中土霉素、四环素等抗生素残留量的HPLC检测方法。流动相为乙腈∶NaH2PO4（0.01mol.L-1）=18∶82,流速为1mL.min-1,检测波长为355nm。该方法测土霉素和四环素的相对标准偏差RSD分别为1.7%和3.3%,加样回收率在95%~105%之间。该方法准确、快捷、稳定,可用于水产品中四环素类抗生素残留量的分析检测。HPLC method for determination of residual tetracycline antibiotics including oxytetracycline and tetracycline in the aquatic product was established in this work.The mobile phase of acetonitrile∶NaH2PO4（0.01 mol·L-1）was 18∶82,flow rate was 1mL·min-1,the detective wavelength was set as 355nm.RSD for oxytetracycline and tetracycline were 1.7% and 3.3%,respectively,the recovery was within 95%～105%.The method is accurate,convenient and stable,which can be used for analysis of the residual tetracycline antibiotics in aquatic products.

关 键 词：抗生素 HPLC 相对标准偏差 加样回收率 

分 类 号：O657.7[理学—分析化学]