6319例高危型人乳头瘤病毒核糖核酸定量检测的结果分析  

Detection of High Risk Human Papilloma Virus in 6319 Cases

作　　者：南京柱[1] 李秀娟 杨秀[1] 童红莉[1] 李海潮[1] 田亚平[1] 

机构地区：[1]解放军总医院生化科,北京100853 [2]新华医院检验科,北京101100

出　　处：《标记免疫分析与临床》2012年第2期74-77,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨女性高危型人乳头瘤病毒(HPV)感染亚型分布情况及年龄特点。方法采用双通道实时荧光定量PCR检测方法,对6319例女性高危型HPV-DNA 16型、HPV-DNA 18/45型、HPV-DNA 31型、HPV-DNA 33/52/58/67型进行定量分析,比较不同年龄组HPV-DNA阳性率及不同型别HPV-DNA的分布。结果 6319例女性中检出HPV-DNA阳性患者794例,总阳性率为12.6%。51～60岁组与≥61岁组的阳性率均为15.8%,且高于其他年龄组差异有统计学意义(P<0.05),其次为21～30岁组阳性率为13.8%。不同类型HPV-DNA阳性率从高到低依次为HPV-DNA 33/52/58/67型(69.3%)、HPV-DNA 16型(21.5%)、HPV-DNA 18/45型(12.1%)、HPV-DNA 31型(6.9%)。结论高危型人乳头瘤病毒核糖核酸定量检测可用初筛女性宫颈癌的人群,更应作为年龄在38～58岁的女性健康体检时的必选项目,且对宫颈癌的诊断和药物治疗具有重要意义。Objective To investigate the genotype and age distribution of high risk papilloma virus（HPV） infection in female.Methods The HPV-DNA genotypes HPV-DNA 16,HPV-DNA 18/45,HPV-DNA 31 and HPV-DNA 33/52/58/67 in 6319 cases were detected by dual-channel real time fluorescence quantitative PCR,and the high risk HPV-DNA genotypes of positive rate and distribution in different age groups were analyzed.Results 794 cases positive samples were detected in 6319 female with a positive rate of 12.6%.The age 51-60 group and ≥61age group had same positive rate of 15.8% and significantly higher than that of the other groups（P0.05）.The age 21-30 group had the secondly highest positive rate（13.8%）.Different high risk genotypes of positive rate from high to low was HPV-DNA 33/52/58/67（69.3%）,HPV-DNA 16（21.5%）,HPV-DNA 18/45（12.1%） and HPV-DNA 31（6.9%）.Conclusion High risk HPV-DNA testing is an effective screening method for cervical cancer.It should be a project of required to choose in health examination for female in the age from 38.2-57.6,and it has great significance in diagnosis and treatment of cervical cancer.

关 键 词：高危型 人乳头瘤病毒 宫颈癌 双通道 实时荧光定量PCR 

分 类 号：R373[医药卫生—病原生物学] R737.33[医药卫生—基础医学]