叉尾斗鱼遗传多样性的RAPD分析  

Analysis of genetic diversity of paradisefish Macropodus opercularis using RAPD markers

作　　者：刘宏毅[1,2] 黎明星[1,2,3] 肖俊[1,2,3] 黄愉淋[1,2,3] 张明[1,2] 甘西[3] 

机构地区：[1]广西大学亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [2]广西大学动物繁殖研究所,广西南宁530004 [3]广西水产研究所广西水产遗传育种与健康养殖重点实验室,广西南宁530021

出　　处：《大连海洋大学学报》2012年第2期158-161,共4页Journal of Dalian Ocean University

基　　金：广西自然科学基金资助项目(桂科自0991008/Z);中国博士后科学基金资助项目(20110490867);广西壮族自治区直属公益性科研院所基本科研业务费资助项目(2060302 CXIF-2011-04)

摘　　要：采用RAPD标记技术对叉尾斗鱼Macropodus opercularis 5个地理群体(南宁群体、桂林群体、龙岩群体、福州群体和广州群体)的遗传多样性进行了检测。结果表明:从40个随机引物中筛选出11个有效引物,对每条叉尾斗鱼的基因组DNA进行扩增,共扩增出139条片段;各群体的多态位点比例为26.44%～40.95%,Nei基因多样性指数为0.1042～0.1457,Shannon指数为0.1543～0.2176;而总体的多态位点比例、Nei基因多样性指数和Shannon指数分别高达84.89%、0.3110和0.4606。研究表明,叉尾斗鱼的遗传变异主要来自群体间,而群体内部的遗传多样性水平较低。Random amplified polymorphic DNA（RAPD） technique was employed to detect the genetic variations in paradisefish Macropodus opercularis populations distributed in five geographical colonies.In this study,11 effective primers out of 40 random primers were selected to amplify genomic DNA,resulting in 139 repeatable RAPD bands.The 5 populations were shown to have polymorphism frequency of 26.44%-40.95%,Shannon genetic diversity index of 0.1042-0.1457 and Nei genetic diversity of 0.1543-0.2176.On the average,all the population showed the frequency of polymorphism of 84.89%,Nei genetic diversity of 0.3110 and Shannon genetic diversity index of 0.4606,indicating that the genetic variation of the paradiselfish was not from the intra-populations but from inter-populations.

关 键 词：叉尾斗鱼 遗传多样性 随机扩增多态DNA 

分 类 号：Q951[生物学—动物学]