诺如病毒性胃肠炎快速检测方法的研究  

Evaluation of a rapid and efficient method for detecting norovirus infection

作　　者：吕虹[1] 高志勇[2] 张国军[1] 闫惠平[3] 康熙雄[1] 

机构地区：[1]首都医科大学附属北京天坛医院实验诊断中心,100050 [2]北京市疾病预防控制中心传染病与地方病防治所,北京100013 [3]首都医科大学附属北京佑安医院感染与免疫中心,北京100069

出　　处：《首都医科大学学报》2012年第2期148-152,共5页Journal of Capital Medical University

基　　金：国家高技术研究发展计划(863计划)(2008AA02Z416)~~

摘　　要：目的探讨反转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)、转录反转录协同实验(transcription reverse concerted,TRC)在诺如病毒(norovirus,NVs)性胃肠炎检测中的应用,为NVs检测提供更快捷的检测方法。方法收集2007年2月～2007年3月北京地区各医院及各区疾控中心病毒性胃肠炎患者粪便样本364例,分别采用RT-PCR方法和TRC方法进行NVs核酸检测,并以RT-PCR方法为标准对TRC方法进行评价,计算其灵敏度、特异性等指标;通过一致性Kappa检验,评价两种方法的一致性。结果应用TRC方法检测364例病毒性胃肠炎患者粪便样本,NVs检测总阳性率为43.96%(160/364);其中,NVsⅠ型(genegroupⅠ,GGⅠ)阳性率3.85%(14/364),NVsⅡ型(genegroupⅡ,GGⅡ)阳性率40.11%(146/364);在阳性样本中,78.58%的NVs GGⅠ和89.73%的NVs GGI在20min内检出,明显缩短了检出时间。其中男女两组差异无统计学意义(χ2=0.465,P=0.495)。按年龄分组,其中阳性率最高的为50～59岁组,其阳性率为63.63%,阳性率最低的为10～19岁组,其阳性率为18.93%,通过χ2检验,除10～19岁组检出率低于各组外,其余各组间检验结果差异无统计学意义。结论与RT-PCR方法相比,TRC方法检测NVs敏感度高、特异性强,操作简便易行,适用性强。Objective To find a quick and efficient method to detect norovirus（NV） by comparing different approaches using reverse transcription-polymerase chain reaction（RT-PCR） and transcription reverse transcription concerted（TRC）.Methods From February to March 2007,the fecal specimens were collected from 364 sporadic cases of acute viral gastroenteritis in Beijing area.RT-PCR and TRC were used to detect human NVs in stool specimens;RT-PCR was performed to evaluate the results by TRC and analyzed the sensitivity and specificity of results by TRC.The consistency of TCR was evaluated with RT-PCR by kappa test.Results A total of 160 positive cases were detected by TRC out of 364 stool specimens collected from infants and young children or adults with acute viral diarrhea,and NV positive rate was 43.96%,among which 3.85%（14/364） were identified as infection by NV GGI and 40.11%（146/364） as infection with NVs GGⅡ.In positive cases,78.58% NVs GGI and 89.73% NVs GGⅡ were confirmed within 20 minutes.Men and women had no significant difference in the percentage of HuCV infection.The highest positive rate（63.63%） was found in group aged from 50 to 59.The lowest positive rate（18.93%） was found in the group aged from 10 to 19.No significant difference was seen among age groups except for the group aged from 10 to 19.Conclusion By comparing with RT-PCR,TRC presented a good sensitiveness and specificity which seem to be more convenient and time saving,indicating a good broader application.

关 键 词：诺如病毒 转录反转录协同实验 反转录聚合酶链反应 

分 类 号：R44[医药卫生—诊断学]