miRNA-34c对肺腺癌A549细胞的辐射增敏效应  

The radiation sensitization effect of miRNA-34c on A549 cell

作　　者：肖海楠[1] 段广新[1] 张燕娟[1] 周新文[1] 

机构地区：[1]苏州大学医学部放射医学与防护学院放射毒理学教研室江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2012年第2期157-160,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(81071878)

摘　　要：目的研究肺腺癌A549细胞中miRNA-34c的辐射增敏效应。方法单独转染miRNA-34c序列或单独照射或转染和照射联合处理细胞后,采用MTT比色法检测细胞生长抑制率,流式细胞术检测细胞周期,藻红B染色法观察细胞凋亡率,Western blot检测p53蛋白的表达。结果转染联合照射处理组的抑制率及凋亡率都较单独转染或单独照射组明显增高(均P<0.05),且随miR-34c转染浓度的增加而增高(均P<0.05)。细胞周期检测结果显示,细胞明显阻滞于G1/G0期(P<0.05)。联合处理的细胞p53蛋白表达量较单独照射组增加,且呈miRNA-34c浓度依赖性增加(P<0.05)。结论 miRNA-34c对肺腺癌细胞A549有辐射增敏效应,可强化p53蛋白的表达,诱导细胞G1/G0期阻滞和凋亡。Objective To study the radiation sensitization effect of miRNA-34c on A549 cell Methods The cells were divided into transfection, transfection-irradiation and irradiotion groups. The trarsfection group was transfected with miRNA-34c series. The transfection-irradiation and irradiation groups were irradiated with or without miRNA-34c transfection respectively. Then cell growth inhibition ratio was measured by MTT, cell cycle was detected by FCM, cell apoptosis ratio was observed by Erythro- sin B staining and p53 expression was visulized by Western blot. Results The inhibition ratio and apop- tosis ratio of the group with transfection-irradiation combined treatment was apparently higher than those treated by transfection or irradiation and rose as the transfection density increases （ P 〈 0.05 ）. The cell cycle analysis showed that the cell was obviously blocked in G1/G0 Phase. Compared with the irradiation group, the p53 protein expression of the cell with combined treatment increased in a dose dependent ma- ner. Conclusion The miRNA-34c has the radiation sensitization effect on A549 adenocarcinoma cell, through enhancing the expression of p53 protein and inducing the cell cycle blockade and apoptosis.

关 键 词：miRNA-34c 辐射增敏 A549细胞 

分 类 号：R818.05[医药卫生—放射医学]