纳米金-表阿霉素复合体的体外抗肿瘤作用  

In Vitro Antitumor Effects of Epirubicin-nanogold Compounds

作　　者：赵晓旭[1] 潘运龙[1] 胡杨志[1] 覃莉[2] 丁晖[1] 巫青[1] 

机构地区：[1]暨南大学附属第一医院普外科,广东广州510630 [2]暨南大学医学院组织胚胎学教研室,广东广州510632

出　　处：《中山大学学报（医学科学版）》2012年第2期172-177,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家基础研究973计划项目(2010CB833603);国家自然科学基金资助项目(30772131);广东省自然科学基金资助项目(8151063201000034)

摘　　要：【目的】观察纳米金-表阿霉素复合体(EPI-AuNP)能否抑制人脐静脉内皮细胞(HUVEC)、人肝癌细胞(HepG2)的增殖。【方法】采用化学合成法制备EPI-AuNP,通过紫外-可见吸收光谱、荧光淬灭实验、动态光散射及Zeta电位变化对其进行鉴定。体外实验分为AuNP处理组、EPI处理组、EPI-AuNP处理组和空白对照组。将HUVEC、HepG2细胞分别接种于96孔板,培养24 h后各组分别加入AuNP溶液、EPI溶液、EPI-AuNP溶液和无血清培养液200μL,继续培养24 h后:MTT比色法检测HUVEC、HepG2细胞生存率;紫外-可见分光光度法检测各细胞内EPI的积聚量。【结果】紫外-可见吸收光谱显示:AuNP的最大吸收峰在520 nm处,而EPI-AuNP在525 nm处。EPI(100 mg/L)荧光强度为195.2±7.5;EPI-AuNP为16.4±5.0,P=0.000。AuNP的平均粒径及Zeta电位分别为:(14.34±0.75)nm、(-21.19±0.64)mV;EPI-AuNP为:(18.54±1.84)nm、(-15.34±0.72)mV,P<0.01。体外实验:MTT比色法结果显示EPI处理组HUVEC、HepG2细胞的生存率分别为(29.25±1.59)%、(71.10±4.16)%;EPI-AuNP处理组:(21.29±1.51)%、(43.82±2.21)%,P=0.000。【结论】成功合成EPI-AuNP复合体,体外实验证实其对HUVEC、HepG2细胞均具有增殖抑制作用。【Objective】 To observe the in vitro antitumor effects of epirubicin-nanogold compounds（EPI-AuNP）.【Methods】 EPI-AuNP was prepared by chemosynthesis and investigated using UV-Vis spectrophotometer,fluorescence studies,dynamic light scattering,and zeta potential.Human umbilical vein endothelial cells（HUVEC） and HepG2 cells were divided into 4 groups： AuNP treatment group,EPI treatment group,EPI-AuNP treatment group and control group.After seeded in 96-well plate and cultured for 24 h separately,HUVEC and HepG2 cells were treated with 200 uL of AuNP,EPI,EPI-AuNP,and serum-free medium,respectively.Inhibition effect of each group on the HUVEC and HepG2 cells was assessed using MTT colorimetric method.UV-Vis spectrophotometer was applied to detect the cells epirubicin accumulation of different groups.【Results】 A red shift in the SPR band maxima in the EPI-AuNP spectrum（λmax～525 nm） as compared with the spectrum of AuNP alone（λmax～520 nm）;The fluorescence intensity of EPI（100 mg /L） was（195.2 ± 7.5） and EPI-AuNP was（16.4 ± 5.0）,P=0.000.The hydrodynamic diameter of AuNP was（14.34 ± 0.75） nm while EPI-AuNP was（18.54 ± 1.84） nm.Meanwhile,the zeta potential of AuNP was（-21.19 ± 0.64） mV while EPI-AuNP was（-15.34 ± 0.72） mV,P0.01.The HUVEC survival rate of EPI-AuNP treatment group [（21.29 ± 1.51）%] was lower than the EPI group [（29.25 ± 1.59）%].The HepG2 cells survival rate of EPI-AuNP treatment group [（43.82 ± 2.21）%] was lower than the EPI group [（71.10 ± 4.16）%],P0.01.【Conclusions】 EPI-AuNP has been synthesized and indicated enhanced drug potency in vitro by MTT assay.

关 键 词：纳米金-表阿霉素复合体 HUVEC HEPG2细胞 

分 类 号：R453.9[医药卫生—治疗学]