活血解毒益气方含药血清对人脐静脉内皮细胞分泌ICAM-1、MCP-1的影响  

Effect of Huoxuejieduyiqifang on the Expression of ICAM-1 and MCP-1 of Human Umbilical Vein En dothelial Cells

作　　者：彭哲[1] 祝光礼[1] 刘昭[1] 行俊柱 王春林 

机构地区：[1]杭州市中医院心内科,杭州310007 [2]杭州市余杭区第二医院 [3]浙江省衢州中医院

出　　处：《浙江中西医结合杂志》2012年第4期251-253,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：杭州市科技发展计划项目(No.2005633Q18)

摘　　要：目的:探讨经皮外周血管腔内成形术(PTA)后活血解毒益气方药物血清对人脐静脉内皮细胞(HUVECs)分泌ICAM-1、MCP-1的影响。方法:新西兔35只,随机分为正常组、模型组、普伐他汀组及活血解毒益气方大、小剂量组,各7只。除正常组外,另四组首先运用球囊原位扩张、拉伤致兔颈总动脉PTA术后内皮损伤模型。术前3天各组予相应药物给药,造模成功后继续给药15天,获取模型动物和给药动物血清;采用ELISA法观察药物血清作用24h和48h,对人脐静脉内皮细胞分泌ICAM-1、MCP-1活性的影响。结果:活血解毒益气方大、小剂量组和普伐他汀组药物血清作用24h均能显著抑制HUVECs分泌ICAM-1,与模型组比较,P<0.05;血清作用48h,普伐他汀组、大小剂量组仍具有显著的抑制作用,P<0.05。各组药物血清作用24h、48h均能显著降低HUVECs分泌MCP-1,与模型组比较差异有统计学意义,P<0.05。结论:活血解毒益气中药可通过抑制HU VECs分泌ICAM-1、MCP-1,抑制炎症反应。Objective： To evaluate the effect of Huoxuejieduyiqifang on the expression of ICAM-1 and MCP-1 of human umbilical vein endothelial cells（HUVECs）. Methods： Thirty-five New Zealand rabbits were randomly di- vided into normal control group, model group, pravastatin group, and high-and low-dose Huoxuejieduyiqifang groups,7 in each. Endothelial injury was established by percutaneous transluminal coronary angioplasty（PTCA） in other 4 groups except the normal control group. To obtain the mediated serum,the pravastain and Huoxuejieduyiq- ifang groups were mediated with the corresponding drugs for 3 days before PTCA and 15 days after PTCA,and the normal control and model groups were given equivalent saline for the same period. Then HUVECs were cul- tured with the mediated sera, respectively, and the expressions of ICAM-1 and MCP-1 were assessed by ELISA in 24 h and 48 h after intervention. Results： Compared with the model group,the expression of ICAM-1 in the pravastatin and high- and low-dose Huoxuejieduyiqifang groups was significantly decreased in 24 h and 48 h after intervention（both P〈0.05）. Conclusion： Huoxuejieduyiqifang can attenuate inflammation by inhibiting the expression of ICAM-1 and MCP-1 of HUVECs.

关 键 词：PTCA 活血解毒益气方 ICAM-1 MCP-1 人脐静脉内皮细胞 

分 类 号：R285.5[医药卫生—中药学]