姜黄素对Lewis肺癌小鼠TGF-β1、Smad3、Smad7表达的影响  

Effect of Curcumin on the Expression of TGF-β1,Smad3 and Smad7 in Mice with Lewis Lung Cancer

作　　者：杨维泓[1] 姚庆华[1] 徐玉芬[2] 李妍[2] 

机构地区：[1]浙江省中医院,杭州310000 [2]浙江中医药大学

出　　处：《浙江中西医结合杂志》2012年第4期259-261,F0002,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2008CB009)

摘　　要：目的:观察姜黄素对Lewis肺癌小鼠瘤体组织TGF-β1、Smad3、Smad7蛋白表达的影响。方法:雄性C57BL/6小鼠20只,随机分成姜黄素处理组与模型组,每组10只。采用皮下接种瘤细胞悬液造模。造模24h后,模型组与姜黄素组分别腹腔注射注射用水和200mg/(kg.d)浓度的姜黄素混悬液,0.5mL/只,1天1次,连续12天。应用免疫组织化学显色技术,检测小鼠瘤体组织内TGF-β1、Smad3、Smad7蛋白的表达及定位情况。结果:①与模型组比较,姜黄素抑瘤率29.96%。姜黄素组瘤体平均质量(1.64±0.32)g,与模型组(2.34±0.70)g比较,差异具有统计学意义(P<0.05)。②本研究采用免疫组化定量公式计算蛋白表达阳性单位,结果显示TGF-β1染色强度姜黄素组为(17.83±3.46),模型组为(25.76±4.72),两组比较差异具有统计学意义(P<0.01);Smad3染色强度姜黄素组为(14.64±2.24),模型组为(23.71±3.07),两组比较差异有统计学意义(P<0.01);Smad7染色强度结果显示姜黄素组为(10.97±1.89),模型组为(8.46±1.04),两者比较差异具有统计学意义(P<0.01)。③瘤体组织内TGF-β1与Smad3蛋白表达具有相关性(P=0.043)。结论:姜黄素能抑制肿瘤生长,其作用机制可能与上调Smad7蛋白表达,下调Smad3蛋白表达,从而减少TGF-β1的过度表达有关。Objective：To observe the effects of curcumin on the expression of TGF-β1,Smad3,Smad7 proteins in mice with Lewis lung cancer.Methods：Twenty male C57BL/6 mice were randomly divided into model group and curcumin group,10 in each.A mouse model of Lewis lung cancer was established by subcutaneously inocu lating tumor cell suspension.Mice in the model group were intraperitoneally injected with 0.5 ml of saline once a day and 200 mg/kg/d of curcumin suspension was injected once a day in the curcumin group,both for 12 days.Tumor mass was harvested on the thirteenth day after 12 hour fasting.The expression of TGF-β1,Smad3,Smad7 proteins in tumor tissues were assessed by immunohistochemistry.Results：①Curcumin inhibition rate was 29.96%.The average weight of subcutaneous tumor in the curcumin group was 1.64 ± 0.32 g,significantly lighter than that of the model group（2.34±0.70 g）,P 0.05.②The staining intensities of TGF-β1 and Smad3 in the curcumin group were 17.83±3.46 and 14.64±2.24 as compared to 25.76±4.72 and 23.71±3.07 in the model group（P 0.01）.The staining intensity of Smad7 was 10.97±1.89 in the curcumin group,while 8.46±1.04 in the model group,with a significant difference between them（P 0.01）.③The expressions of TGF-β1 and Smad3 pro teins in the tumor tissue was correlated（P =0.043）.Conclusion ：Curcumin can inhibit tumor growth by reducing the excessive expression of TGF-β1 as a result of upregulating Smad7 and downregulating Smad3.

关 键 词：小鼠 Lewis 肺癌 TGF-Β1 SMAD3 SMAD7 姜黄素 

分 类 号：R285.5[医药卫生—中药学] R734.2[医药卫生—中医学]