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作 者:张敏[1,2] 郭素英[1] 李娟[1] 秦松[3] 蔡敬民[1]
机构地区:[1]合肥学院生物与环境工程系,合肥230022 [2]中国科学院研究生院生命科学学院,北京100049 [3]中国科学院海洋研究所,青岛266000
出 处:《生物学杂志》2012年第2期31-33,54,共4页Journal of Biology
基 金:中国科学院海洋863子课题;安徽省优秀青年基金(编号04043051);合肥学院科研基金(编号04ky006zr)资助
摘 要:对海洋放线菌H-109菌株产生的桃红色色素的理化性质进行了研究。结果表明,丙酮为最佳浸提剂,浸提时间为1 h;最大吸收波长为530 nm;对自然光和紫外光有较好的稳定性;温度对不同浓度色素的影响不同,相对低浓度时(A530值0.1)较稳定;相对高浓度(A530值大于0.24)时,随着温度升高,有胶状沉淀析出;pH值对色素稳定性有影响,pH值7~8时较稳定,pH值小于3时,为亮黄色;pH值大于10时,有紫红色沉淀析出,该沉淀可以重溶于丙酮。Physi-ehemical characteristics of natural persicineus pigment produced by a strain of marine actinomyces H-109 were investigated. The pigment could be extracted after being cultivated 5 days at 28℃. The pigment was dissolved in different reagents. The re- sults showed that the optimal extracting reagent was acetone for more than 1 h. The pigment was quite stable to the sunlight or UV. The effects of temperature on pigment of different concentration were dill)rent. The low concentration pigment (A530 value about 0. 1 ) was stable in water at temperature lower than 100℃, while the high concentration pigment (A530 about 0. 2) was unstable in the corresponding temperature with the increasing temperature. The effect of changed pH on the stability of pigment was strong. Its solution colour was bright yellow at pH 〈 3 while it was purple precipitation at pH 〉 10.
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