转化生长因子β1/结缔组织生长因子通路在强直性脊柱炎中的表达  

Expression of transforming growth factor beta 1 and conection tissue growth factor in ankylosing spondylitis

作　　者：王庆文[1] 曾沛英[1] 蔡月明[1] 陈澄[1] 路晓燕[1] 蓝辉耀[2] 

机构地区：[1]北京大学深圳医院风湿免疫科,深圳518000 [2]香港中文大学内科及药物治疗学系,李嘉诚健康科学研究所,中国香港特别行政区

出　　处：《北京大学学报（医学版）》2012年第2期244-249,共6页Journal of Peking University:Health Sciences

基　　金：广东省科技计划项目(2009B030801071);广东省医学科研基金(2009619)资助~~

摘　　要：目的:了解强直性脊柱炎(ankylosing spondylitis,AS)骶髂关节中转化生长因子β1(transforming growthfactor beta 1,TGF-β1)/结缔组织生长因子(connective tissue growth factor,CTGF)通路的主要分子表达情况,探讨TGF-β1/CTGF通路与AS关节纤维化的关系。方法:30例AS患者均通过酶联免疫吸附(enzyme-linked immunosor-bent assay,ELISA)法检测血清TGF-β1、CTGF水平,通过免疫组织化学方法检测TGF-β1、p-smad3、smad7、CTGF、Ⅰ型胶原和Ⅲ型胶原的表达。结果:与正常对照相比,AS骶髂关节组织可见TGF-β1、CTGF高表达,主要在骨髓及血管翳中炎症细胞的胞浆中表达;smad7明显低表达,p-smad3则主要表达于骨髓及血管翳中炎症细胞的细胞核中,提示smad3已被激活;Ⅰ型胶原和Ⅲ型胶原大量沉积,但血清中的TGF-β1和CTGF的水平与正常对照组间差异无统计学意义。结论:AS骶髂关节存在TGF-β1的过高表达,smad信号通路的激活,samd7的低表达,导致CTGF高表达,Ⅰ型胶原和Ⅲ型胶原大量沉积。Objective：To investigate the expressions of transforming growth factor beta 1（TGF-β1） /smad,connective tissue growth factor（CTGF）,collagenⅠand collagen Ⅲ in ankylosing spondylitis（AS）.Methods： Thirty patients with AS were included in the study.All the patients were performed with computed tomography-guided needle biopsy in sacroiiliac joint.Sera TGF-β1 and CTGF were determined by enzyme-linked immunosorbent assay（ELISA）.Immunohistologic studies were performed with the alkaline phosphatase-anti-alkaline phosphatase technique to assess the expressions of TGF-β1,p-smad3,smad7,CTGF,collagenⅠand collagen Ⅲ in sacroiiliac joint tissue samples.Results： In the AS patients,neither serum TGF-β1 level nor serum CTGF level was found significantly different from that of the controls [（6.7±2.1）mg/L vs.（5.4±5.8）mg/L,P0.05,（0.83±0.46）μg/L vs.（1.07±0.79）μg/L,P0.05].In contrast to the healthy controls,TGF-β1 and CTGF were found upexpressed in cytoplasm of inflammatory cells in pannus and bone marrow in sacroiliac tissue samples of patients with AS [（104.5±66.2） /HP vs.（24.4±9.3） /HP,（57.94±42.40） /HP vs.（2.67±2.52） /HP].Meantime,p-smad3 was found expressed in the nuclear,while smad7 was detected to be downexpressed.Additionally,collagenⅠand collagen Ⅲ were found upexpressed in bone,cartilage and ligament tissue.Conclusion： TGF-β1,CTGF,collagenⅠand collagen Ⅲ were upexpressed in sarcoiliac joints of AS patients.TGF-β1/CTGF may play an important role in articular cartilage fibrosis and ossification of AS by smad signal pathyway.

关 键 词：脊柱炎 强直性 转化生长因子Β 结缔组织生长因子 

分 类 号：R593.23[医药卫生—内科学]