响应曲面法优化中药栀子中西红花苷类成分的提取工艺  

Optimization of extraction parameters of crocin from Gardeniae Fructus using response surface methodology

作　　者：张珊[1] 毕志明[1] 张雪[1] 范琪[1] 赵芳[1] 

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,南京210009

出　　处：《中国新药杂志》2012年第8期917-920,共4页Chinese Journal of New Drugs

基　　金：教育部新世纪优秀人才支持计划项目(NCET-06-0497);江苏省大学生实践创新训练计划项目(J0630858)

摘　　要：目的:优化栀子中西红花苷类成分的提取工艺。方法:以西红花苷-I和西红花苷-II的提取率为指标,运用HPLC色谱分析方法,采用中心星点设计-响应曲面法对提取工艺条件进行优化。结果:优化得到栀子的最佳提取参数为乙醇浓度51%,提取温度65℃,提取时间18 min。验证试验结果与预测值偏差分别为1.51%,1.38%。结论:该提取工艺稳定、合理、预测性良好。Objectives： To optimize the process of extracting crocins from Gardenia Fructus.Methods： HPLC,central composite design and response surface methodology were used to optimize the process of crocin-1 and crocin-2 extraction.Results： The optimal extraction parameters were as follows： ethanol concentration,51%;extraction temperature,65 ℃ and extraction time,18 min.The deviations between the experimental results and predicted values of crocin-1 and crocin-2 rates were 1.51% and 1.38%,respectively.Conclusion： The extraction technology is reliable,reasonable and highly predictive.

关 键 词：栀子 西红花苷类 响应曲面法 

分 类 号：R932[医药卫生—生药学]