透皮促进剂对白花前胡甲素体外经皮渗透的影响  

Influence of penetration enhancers on in vitro transdermal permeation of dl-praeruptorin A

作　　者：张贤华[1,2] 串星星[2] 洪晓翔[1] 金梅花[3] 朱铉[2] 洪颖[2] 孟飞宏[2] 

机构地区：[1]厦门大学附属中山医院演武分院药剂科,厦门361005 [2]厦门大学药学院,厦门361005 [3]延边大学医学部基础医学院生物化学与分子生物学教研室,延吉133002

出　　处：《中国新药杂志》2012年第8期926-930,共5页Chinese Journal of New Drugs

基　　金：厦门市科技计划项目(3502Z20103009);厦门市科技计划项目(3502Z20093012)

摘　　要：目的:考察透皮促进剂对白花前胡甲素(dl-praeruptorin A,Pd-Ia)体外经皮渗透的影响。方法:采用改进的Franz扩散池,以大鼠离体皮肤为渗透屏障,用高效液相色谱法对Pd-Ia进行含量测定,考察月桂氮酮(Azone)及1%Azone与不同浓度丙二醇(PG)混合物对Pd-Ia透皮吸收的影响。结果:使用Azone对Pd-Ia有促透作用,1%Azone效果较好,平均渗透速率达到4.064μg.cm-2.h-1;1%Azone与15%PG合用促透效果最好,平均渗透速率达到4.889μg.cm-2.h-1,且与单用1%Azone有显著性差异(P<0.05)。结论:1%Azone与15%PG合用时,含0.5%Pd-Ia溶液体外渗透具有最大促透效果,体现出协同作用。Objective： To study the effect of penetration enhancers on the in vitro transdermal permeation of dl-praeruptorin A（Pd-Ia） through rat skin.Methods： The permeation experiments were evaluated by Franz diffusion cell in excised rat skin.HPLC-UV method was established to determine the concentration of Pd-Ia in receptor compartment.Azone or 1% Azone combined with propylene glycol（PG） as chemical penetration enhancers were applied to study the skin permeation rates of Pd-Ia.Results： 1% Azone alone achieved the highest penetration rate,4.064 μg·cm-2·h-1.1% Azone combined with 15% PG was the best penetration enhancer with the maximal penetration rate of 4.889 μg·cm-2·h-1.Conclusion： Combination of 1% Azone with 15% PG is effective on transdermal permeation of Pd-Ia in vitro,and the combination exerts a synergistic effect.

关 键 词：白花前胡甲素 经皮渗透 促进剂 月桂氮酮 丙二醇 

分 类 号：R94[医药卫生—药剂学]