电针对帕金森病模型大鼠黑质神经干细胞巢蛋白、PCNA和β-微管蛋白表达的作用。  

Effect of electroacupuncture on Nestin, PCNA and Tujl expression in neural stem cells of substantia nigra in the Parkinson＇s disease model rats

作　　者：倪进忠[1] 丁艳霞[1] 熊克仁[1] 

机构地区：[1]皖南医学院解剖学教研室,芜湖241002

出　　处：《解剖学杂志》2012年第2期181-185,共5页Chinese Journal of Anatomy

基　　金：安徽省高校省级自然科学项目（KJ20112392）

摘　　要：目的：研究不同频率和疗程电针对帕金森病（PD）模型大鼠黑质致密部（SNc）神经于细胞（NSC）表达的影响。方法：SD大鼠随机分为16组，正常对照组，1、2、3周假手术组、模型组，1、2、3疗程美多巴组、低频电针组、高频电针组，采用右侧纹状体内注射6-羟基多巴胺（6-OHDA）制备PD模型，取合谷和太冲穴，分别给予低频（2Hz）和高频（100Hz）电针治疗。免疫组织化学观察黑质致密部的巢蛋白、PCNA和p微管蛋白（Tujl）表达。结果：PD模型大鼠黑质致密部的巢蛋白、PC-NA和Tujl表达增加，高频电针可增加其巢蛋白、PCNA和Tujl表达，低频电针对其没有影响。结论：高频电针可增加黑质致密部自体NSC的增殖，并诱导其向神经元前体细胞分化。Objective： To explore the expression changes of proteins related neural stem cells （NSC） in the substantia nigra pars compacta （SNc） in Parkinson＇s disease （PD） model rats treated with different frequency and course of electroacupuncture （EA）. Methods： Ninty-six Sprague-Dawley rats were randomly divided into sixteen groups：a normal control group; 1-, 2- and 3- week sham-operated groups, a model group; 1-, 2- and 3- course of Madopar treatment groups, a low-frequency EA treatment group, and a high-frequency EA treatment group. The PD model was prepared by injection of 6-hydroxydopamine （6-OHDA） into the right striatum. ＂Hegu＂ and ＂Taichong＂ were given low-frequency （2 Hz） and high-frequency （100 Hz） EA treatment. Immunohistochemistry was used to observe the Nestin, proliferating cell nuclear antigen （PCNA） and β-tubulin isotype-Ⅲ （Tujl） expression in SNc. Results： The expression of Nestin, PCNA, Tuj I increased in SNc of the PD model rats. The high-frequency EA improved its Nestin, PCNA and Tujl expression, while the low-frequency EA had no effect. Conclusion： High-frequency EA can improve the autologous NSC proliferation of SNc and induce the differentiation into neuronal precursor cells.

关 键 词：帕金森病 黑质 神经干细胞 电针 

分 类 号：R2-03[医药卫生—中医学]