熊胆粉与其他三种动物胆粉的FT-IR特征分析及药理作用比较  

Characteristic analysis by FT-IR for bile powder from bear and three other animals and comparison of the pharmacological effects

作　　者：鄢海燕[1] 邹纯才[2] 

机构地区：[1]皖南医学院药剂学与生药学教研室,安徽芜湖241002 [2]皖南医学院药物分析与药物化学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2012年第2期109-111,115,共4页Journal of Wannan Medical College

基　　金：安徽高校省级自然科学研究项目(KJ2011Z391)

摘　　要：目的:建立熊胆粉与猪胆粉、鸡胆粉、牛胆粉的IR分析方法并比较镇咳、抗惊厥和解热作用,为寻找熊胆粉的代用品提供依据。方法:采用IR法测定熊胆粉、猪胆粉、牛胆粉和鸡胆粉IR光谱,分析IR特征并采用氨气致咳法、戊四氮致惊厥法和干酵母致热法比较四种胆粉对小鼠的镇咳作用、抗惊厥作用和解热作用。结果:鸡胆粉与熊胆粉的IR图谱基本一致,熊胆粉IR光谱特征为:1 656 cm-1(s)和1 557 cm-1(s),有酰胺基(-CO-NH-)双峰,且峰强大致相等,1 188 cm-1(宽峰,s)和1 051 cm-1(s)为-SO3H双强峰,T1 188 cm-1/2 954 cm-1及T 1 051 cm-1/1 188 cm-1均约为1.0;猪胆粉、牛胆粉或四种胆粉的相互混合品的IR光谱不具备或不同时具备以上三个特征。四种胆粉均具有镇咳、抗惊厥和解热作用,其中鸡胆粉镇咳作用较强,猪胆粉抗惊厥作用最强,而熊胆粉的解热效果较好。结论:IR法可用于鉴别熊胆粉与牛胆粉、猪胆粉,具有操作简单、分析时间短和鉴别特征明显等优点,但不能准确区分熊胆粉与鸡胆粉。四种胆粉均有镇咳、抗惊厥和解热作用,效果有所差异。Objective：To establish an analytical method by Fourier transform infrared spectroscopy（FT-IR） for identification of the bear bile powder and other powder from bile of swine,chicken and ox and compare the antitussive,anticonvulsant and antipyretic effects for alternative basis of bear bile products.Methods：FI-IR was used to identify the spectral characteristics of bile power prepared from bear,swine,chickens and oxen.The animal models with cough,convulsion and fever were induced by ammonia,pentylenetetrazol and dry yeast,respectively,and medicated with the four different preparation to determine the antitussive,anticonvulsant and antipyretic effects.Results：FI-IR spectrum of bear bile powder was almost identical to that of chicken,with spectral characteristics of amide（-CO-NH-） peaks at 1 656 cm-1（s） and 1 557 cm-1（s）,roughly equal intensity,strong double peak of-SO3H at 1 188 cm-1（broad peak,s）and 1 051 cm-1（s）,T 1 188 cm-1/2 954 cm-1 and T 1 051 cm-1/1 188 cm-1 of about 1.0.Single FI-IR spectrum of the powder from porcine gall and ox bile or spectrum for the mixtures of the four were not totally or partially shown the features described above.Although the four kinds of bile powder had concurrent effects on relieving cough,convulsion and fever,chicken bile product appeared best antitussive,convulsion responded well to porcine gall powder,and bear bile preparation showed strongest antipyretic effect.Conclusions：FI-IR spectroscopic method can be used to identify the powder of bear,ox and swine bile with its simple performance,shortened analytic requirement and distinct features in identification,unfortunately,this approach seems incompetent for screening the powder of bear bile from that of chicken gall.In addition,cough,convulsion and fever may respond to the four agents,yet,the effect is slightly different.

关 键 词：动物胆汁 FT-IR法 药理作用 

分 类 号：R28[医药卫生—中药学]