甘蓝型油菜EMS诱变材料的耐湿性鉴定与筛选  

Verification and Identification of Waterlogging Tolerance in Brassica napus Mutated by EMS

作　　者：薛远超[1] 李加纳[1] 刘列钊 徐新福[1] 

机构地区：[1]西南大学南方山地农业教育部工程研究中心,重庆市油菜工程技术研究中心,重庆400716

出　　处：《西南师范大学学报（自然科学版）》2012年第4期76-80,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市科技攻关资助项目(2011GGC018)

摘　　要：该研究以1 132份来自甘蓝型油菜中双9号和GH18的EMS诱变处理自交3代种子为材料,对芽期耐湿性进行综合评价.实验数据表明,EMS诱变后代的耐湿性存在广泛变异.以湿害处理的成苗率为指标进行初筛,有26%以上的材料成苗率高于50%.其中所选材料的5个耐湿性指标平均值均高于未诱变对照,各指标变异系数在0.106 6～0.485 3之间,变异丰富.用相对活力指数对其进行耐湿性分级,分别从GH18和中双9号的EMS诱变后代中筛选得到14个和2个强耐湿性材料,这16份材料可以作为甘蓝型油菜耐湿性育种的优异资源.1132 M3 generation rape seeds which are mutated by EMS including Zhongshuang 9 and GH18,are used in this research and their waterlogging tolerance are comprehensively estimated.The experiment results showed that EMS mutations cause wide variation.Selected by the seedling rate of the materials treated by waterlogging,there are 26 percent of mutations with the seedling rate higher than 50% and of these selected mutations,all the 5 humidity indexes are higher than the contrast index,with coefficient of variation between 0.1066 and 0.4853,thus it is confirmed rich in variation.Graded for the waterlogging tolerance of these treated materials by their relative activity index,14 and 2 materials with strong high waterlogging tolerance were selected from the EMS mutations GH18 and 2 from Zhongshuang 9 respectively and these 16 Brassica materials would be used as excellent resources in waterlogging tolerance breeding.

关 键 词：甘蓝型油菜 EMS诱变 耐湿性 发芽期 

分 类 号：S634.3[农业科学—蔬菜学]