可溶型白细胞介素-13受体α2对成纤维细胞胶原蛋白合成的抑制作用  

Inhibitory Effect of Soluble IL-13 Receptor Alpha 2 on Fibroblast Collagen Synthesis

作　　者：石小玉[1] 熊丽霞[2] 周莹[2] 蔡伟[2] 赵林[3] 章克萍[1] 李文林[2] 

机构地区：[1]南昌大学医学实验教学部 [2]南昌大学基础医学院 [3]江西省医学科学研究所医学生物高技术重点实验室,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第1期1-5,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(30860118)

摘　　要：目的应用可溶型IL-13受体α2(sIL-13Rα2)抑制成纤维细胞胶原合成,探索生物治疗纤维化的新途径。方法构建pMSCV/sIL-13Rα2载体和表达sIL-13Rα2的细胞模型,分子量(0.2×106)滤膜离心浓缩培养液,Ni2亲和层析,SDS-PAGE分析sIL-13Rα2,Western blot检测sIL-13Rα2,RT-PCR和羟脯氨酸法显示成纤维细胞Ⅰ型胶原蛋白的表达。结果构建的重组体pMSCV/sIL-13Rα2成功转染入HEK293细胞,并表达sIL-13Rα2。50μg.L-1 IL-13促进人瘢痕成纤维细胞系CRL-1762生成Ⅰ型胶原蛋白,50μmol.L-1 sIL-13Rα2能有效抑制IL-13刺激CRL-1762细胞合成Ⅰ型胶原蛋白。结论 sIL-13Rα2靶向IL-13抑制成纤维细胞Ⅰ型胶原蛋白的合成。Objective To explore a new biological therapy in fibrosis through inhibiting fibroblast collagen synthesis by soluble IL-13 receptor α 2（sIL-13Rα2）.Methods The pMSCV/sIL-13 Rα2 vector was constructed and cell model expressing sIL-13 Rα2 was established.The medium was concentrated by ultrafiltration（0.2×106-molecular-weigh out off） and sIL-13 Rα2 was analyzed by Ni2 affinity chromatography,SDS-PAGE and Western blot.The expression of type Ⅰ collagen protein in fibroblasts was detected by RT-PCR and hydroxyproline method.Results HEK293 cells were successfully transfected with the constructed recombinant pMSCV/sIL-13 Rα2 and expressed sIL-13 Rα2.Type Ⅰ collagen synthesis was promoted by 50 μg·L-1 IL-13 in scar fibroblast CRL-1762 cells.But the promoting effect of IL-13 was inhibited by 50 μmol·L-1 sIL-13 Rα2.Conclusion Type Ⅰ collagen synthesis can be inhibited by targeting IL-13 with sIL-13 Rα2.in fibroblasts.

关 键 词：成纤维细胞 白细胞介素13受体 Ⅰ型胶原蛋白 

分 类 号：R349.8[医药卫生—基础医学]