汉、回及维吾尔三民族红细胞CR1基因点突变差异的研究  

Changes and the Significance of Genomic Determination of the CR1 Density Polymorphism on Erythrocyte Among Nation Han Hui and Uygur

作　　者：洪炜[1] 马洪滨[1] 朱剑功[1] 刘立明[1] 李永利[1] 王雪飞[1] 王大刚[1] 庞君丽[1] 杨宁[1] 李妍[1] 王海滨[1] 

机构地区：[1]解放军第302医院临床检验医学中心,北京100039

出　　处：《医学研究杂志》2012年第4期102-104,共3页Journal of Medical Research

摘　　要：目的研究汉族、维吾尔族及回族三民族正常人群红细胞补体受体Ⅰ型(ECR1)基因点突变的差异及意义。方法采用PCR和HindⅢ酶切技术分析红细胞CR1分子基因点突变。结果三民族红细胞CR1基因突变率分别为:汉族32.5%,维吾尔族24.0%,回族23.5%,统计学比较虽无显著性差异(χ2=4.62,P>0.05)。三民族女性红细胞CR1基因突变率(32.5%)明显高于男性(23.7%),虽无显著统计学差异(χ2=3.71,P>0.05),但维吾尔族女性突变率(32.8%)显著高于男性(18.3%,(χ2=4.25,P<0.05);汉族男性的红细胞CR1基因多态性分布(31.5%)显著高于维吾尔族(18.3%)和回族(19.6%)男性(χ2=6.10,P<0.05);汉族和回族男女之间红细胞CR1基因多态性分布无显著差异。结论汉族男性红细胞CR1基因突变率显著高于维吾尔族和回族男性,维吾尔族女性突变率显著高于维吾尔族男性。此外,汉族人群红细胞CR1基因突变率有高于维吾尔族和回族的趋势,三民族女性红细胞CR1基因突变率有高于男性的趋势,这对免疫性疾病地域差异的研究具有重要意义。Objective To study the changes and the significance of genomic determination of the CR1 density polymorphism on erythrocyte among the nation Hart, Hui and Uygur. Methods Polymerase chain reaction （PCR） and Hindm restriction enzyme digestion assay of ECR1 were used in this study. Results The spot mutation rate （sMR） of ECR1 density gene in peoples with nation Hun was 32.5% , and the sMR in nation Hui was 23.5% and nation Uygur was 24.0% . There was no significant changes among three nations （X2 = 4.62, P 〉 0.05 ）. The sMR in famale of nations was higher （32.5 % ） than that in male （23.7%）. Though the sMR also had no significance difference between famale and male in three mations（x2=3.71 ,P 〉 0.05 ） , but the sMR of ECRI in female（ 32.8% ） in nation Uygur was significant higher than that in male （18.3% ,X2 = 4.25 ,P 〈 0.05 ） , and the sMR of ECR1 in male of nation Han （31.5%） was significant higher than that in male of nation Hui（ 18.3% ） and nations Uygur（ 19.6% ;x2=6. 1, P 〈 0.05 ）. There was no significant difference between nation Han and nation Hui. Conclusion The sMR of ECR1 in male in nation Han was obviously higher than that in nation Hui and in nation Uygur male, and the sMR of ECRI in nation Uygur female was significant higher than that in nation Uygur male. Though there was no significant difference among three nations in the sMR of ECR1, but the sMR in nation Hart was obviusely higher than that in other two nations, and the sMR of ECRI in female in three nations was higher than that in male. Those finding may play important role in studying the mechanism of immune disesase in different fields.

关 键 词：民族 红细胞 补体一型受体 多态性 

分 类 号：R587.1[医药卫生—内分泌]