磷酸二酯酶4D基因多态性与动脉粥样硬化血栓形成性脑卒中的关系  

Association of Phosphodiesterase 4D Gene with Atherothrombosis Ischemic Strok

作　　者：王汉旻[1] 陈晓丽[2] 叶好好[2] 毕涌[2] 潘东波 徐丽英[1] 吴建波[4] 黄海波[1] 

机构地区：[1]温州医学院附属第一医院脑血管科,325000 [2]温州医学院附属第一医院神经科,325000 [3]温州市第八人民医院 [4]龙湾医科所

出　　处：《医学研究杂志》2012年第4期134-137,共4页Journal of Medical Research

基　　金：温州市科技计划资助项目(Y20060266;Y20100149);鹿城区科技局科技文化项目(S090208)

摘　　要：目的采用病例对照研究探讨中国汉族人群磷酸二酯酶4D(phosphodiesterase 4D,PDE4D)基因与动脉粥样硬化血栓形成性脑卒中(atherothrombosis ischemic stroke,AIS)的关系。方法应用聚合酶链反应—限制性片段长度多态性方法,检测235例浙南地区汉族人群AIS患者和105例非脑卒中对照组PDE4D基因SNP83、32酶切多态性。结果 AIS患者PDE4D基因SNP83 C等位基因频率明显高于对照组,是AIS的危险因素(OR=1.718,95%CI:1.120～2.633;P=0.012);CC+CT基因型亦明显高于对照组,差异有统计学意义(P<0.05),(OR=1.875,95%CI=1.137～3.092);调整糖尿病、HDL-C等危险因素后,差异仍存在(OR=2.023,95%CI:1.207～3.391)。PDE4D基因SNP32位点在两组比较无统计学意义(P>0.05)。结论对于中国汉族人群,PDE4D基因SNP83是AIS的危险因素,而SNP32与AIS无关联。Objective To investigate the association between 2 single nucleotide polymorphisms（SNPs） of the phosphodiesterase 4D （PDE4D） gene and atherothrombosis ischemic stroke （AIS）in the Hau population in China. Methods Two polymorphisms SNP83 and SNP 32 in PDE4D gene were analyzed in 235 patients of AIS and 105 non - cerebral infarction controls from south of Zhejiang Province using polymerase chain reaction - restriction fragment length polymorphism method in a case - control study. Results The C allele frequency of SNP83 in cases was higher than controls. The C allele was a risk factor for AIS （ OR = 1. 718,95% C1 ： 1. 120 - 2. 633 ; P = Genotype frequencies between cases and controls at SNP83 showed significant differences. The（ CC ＋ CT） genotypes had an OR of P = 0. 008） for cases compared with the TT genotype. No association of the SNP32 in PDE4D with A1S was observed in our study. on SNP83 in the PDE4D gene is associated with AIS in the Chinese Hart population.

关 键 词：磷酸二酯酶4D 单核苷酸多态性 脑梗死 遗传 

分 类 号：R543.5[医药卫生—心血管疾病]