腺苷受体激动剂在豚鼠实验性哮喘中的作用  

Effects of inhaling N_6-phenyl-2R-isopropyl-adenosine on the experimental asthmatic guinea-pigs

作　　者：李荣[1] 张珍祥[1] 彭则[1] 

机构地区：[1]武汉同济医科大学附属同济医院呼吸病研究室,湖北武汉430030

出　　处：《中国病理生理杂志》2000年第2期135-138,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :研究腺苷受体激动剂R -苯异丙基腺苷 (N6-phenyl- 2R -isopropyl-adenosine ,R -PIA)对实验性哮喘豚鼠气道阻力、肺顺应性的影响。方法 :复制豚鼠实验性哮喘动物模型 ,给予R -PIA 5mg/mL的生理盐水雾化吸入 ,观察气道阻力、肺顺应性的变化 ,以及肺泡灌洗液中NO-x 、环一磷酸腺苷 (cAMP)、环一磷酸鸟苷 (cGMP)、肿瘤坏死因子 (TNF)含量的变化。结果 :给予R -PIA雾化吸入后较哮喘组气道阻力增加 (P <0 0 5 ) ,肺顺应性下降 (P <0 0 5 ) ,肺泡灌洗液中NO-x 、cAMP、cGMP、TNF水平下降 ,而TNF含量无明显变化。NO哮喘组为 2 39± 0 0 5 ,R -PIA组为 1 34± 0 0 3(P <0 0 5 ) ,cAMP哮喘组为 1 6 2± 0 0 4,R -PIA组为 0 79± 0 0 2(P <0 0 5 ) ,cGMP哮喘组为 0 47± 0 0 9,R -PIA组为 0 2 5± 0 0 2 (P <0 0 5 ) ,TNF哮喘组为 45 18± 17 2 6 ,R -PIA组为 43 6 2± 14 2 9(P >0 0 5 )。结论 :R -PIA有收缩气道的作用 ,并与肺泡灌洗液中NO-x 、cAMP、cGMP水平下降有关。AIM:To evaluate the role of inhaling N 6-phenyl-2R-isopropyl-adenosine (R-PIA) on the airway resistance and pulmonary compliance of experimental asthmatic guinea-pigs. METHODS: Experimental model of asthmatic guinea-pigs were made. Inhaling R-PIA 5 mg/mL( 5 mg R-PIA in 1 mL 0.9% saline). The airway resistance, pulmonary compliance and NO- x, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP),tumor necrosis factor (TNF) in bronchoalveolar lavage fluid (BALF) were observed. RESULTS: Compared with asthma group, after inhaling R-PIA, the airway resistance of experimental asthmatic guinea-pigs increased ( P <0.05), but the pulmonary compliance decreased ( P <0.05). So did the NO- x and cAMP, cGMP in BALF ( P <0.05), TNF in BALF had no change ( P >0 05). CONCLUSION: Inhaling R-PIA could constrict the airway of experimental asthmatic guinea-pigs.

关 键 词：豚鼠 哮喘 动物实验 苯异丙基腺苷 

分 类 号：R562.25[医药卫生—呼吸系统]