磷酸肌酸对大鼠心肌缺氧、复氧心肌线粒体肌酸激酶肌膜型亚基mRNA表达水平的影响  

The influence of phosphocreatine on expression of mitochondrial sarcomeric creatine kinase mRNA of myocardium ischemia and reperfusion in rats

作　　者：李芳 李辉[2] 朱世军[2] 王孝铭[2] 于康振 

机构地区：[1]解放军211医院肾内科,黑龙江哈尔滨150086 [2]哈尔滨医科大学病理生理教研室,黑龙江哈尔滨150086 [3]兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国病理生理杂志》2000年第2期143-145,共3页Chinese Journal of Pathophysiology

基　　金：黑龙江省自然科学基金资助! (D0 96 13)

摘　　要：目的和方法 :以离体心脏灌流法制备大鼠心肌缺氧、复氧模型 ,提取大鼠心肌mRNA并标记心肌线粒体肌酸激酶肌膜型亚基 (sMiMi -CK)基因探针 ,进行Northern杂交 ,观察不同缺氧、复氧时sMiMi -CKmRNA的变化及磷酸肌酸 (PCr)的保护作用。结果 :随着缺氧时间的延长 ,sMiMi-CK的mRNA逐渐减少 ,复氧后sMiMi-CK进一步减少 ,PCr能显著改善sMiMi-CK的表达量。结论 :PCr对缺氧、复氧的心肌具有明显的保护作用。AIM and METHODS: Rat myocardial mRNA was extracted and mitochondrial sarcomeric creatine kinase (sMiMi-CK) was used as labled probed in vitro myocardial ischemia/reperfusion rats. Northern blot was performed to determine the changes of sMiMi-CK mRNA in different periods of ischemia/reperfusion and the protective effect of phosphocreatine (PCr).RESULTS:During early ischemial (10 min) mRNA was decreased about 18 24%. After reperfusion mRNA decreased again. PCr effectively prevented the decrease of mRNA expression of sMiMi-CK. CONCLUSION:The results indicated that PCr may possess protective effect on anoxic and reoxygenated myocardium.

关 键 词：心肌缺血 线粒体 mRNA SMiMi-CK基因 

分 类 号：R542.202[医药卫生—心血管疾病]