两种拟南芥coi1突变体的鉴定与初步分析  

Identification and preliminary analysis of coi1 mutants in Arabidopsis thaliana

作　　者：马天意[1] 李梦莎[1] 石璐[1] 国静[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨150040

出　　处：《黑龙江大学自然科学学报》2012年第2期251-253,268,共4页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(31070351;31100196);中央高校基本科研业务费专项资金资助项目(DL09BA25);中国博士后科学基金资助项目(20100480963);黑龙江省博士后基金资助项目(LBH-Z10278);高等学校博士学科点专项科研基金资助项目(20110062120015)

摘　　要：COI1(coronatine-insensitive 1)蛋白是茉莉酸信号途径的信号开关,coi1突变体是茉莉酸信号调控机制研究中的重要实验材料。对从拟南芥生物资源中心购买的两种COI1(At2g39940)的T-DNA插入突变体SALK-095916和SALK-045434,通过基因型分析筛选获得了纯合突变体。进一步的基因表达检测和茉莉酸敏感性试验表明,两种纯合突变体中COI1基因的表达量分别约为野生型植株的40%和50%,突变体幼苗对茉莉酸的敏感性显著降低。获得的SALK-095916(命名为coi1-22)和SALK-045434(命名为coi1-23)纯合突变体可以作为实验材料在进一步的茉莉酸信号途径机制研究中使用。Coronatine-insensitive 1（coi1） mutant plays a fundamental function in the studies of jasmonic acid（JA） signaling for the on-off switch role of COI1（coronatine-insensitive 1） in JA signaling pathway.Two T-DNA insertion mutants of COI1,SALK-095916 and SALK-045434 were purchased from the Arabidopsis Biological Resource Center（Ohio State University,USA）,and the homozygous mutants were identified by genotyping analysis.Further quantitative real-time RT-PCR analysis and JA-sensitivity analysis showed that the expression of COI1 gene was significantly reduced in both homozygous mutants and the seedlings of the homozygous mutants were insensitive to jasmonic acid（JA）.The homozygous mutants SALK-095916 and SALK-045434,which were renamed coi1-22 and coi1-23 respective can be used as leaky mutants in the future study of JA signaling.

关 键 词：拟南芥 COI1 T-DNA插入突变体 鉴定 

分 类 号：Q786[生物学—分子生物学]