基因枪法转化小麦及转基因植株的获得  

Wheat Transformation by Microprojectile Bombardment and Transgenic Wheat Regeneration

作　　者：于晓红[1] 朱祯[1] 徐鸿林[1] 朱玉[1] 吴茜[1] 高越峰[1] 付志明[1] 肖桂芳[1] 安利佳 李向辉[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《高技术通讯》2000年第2期13-17,共5页Chinese High Technology Letters

基　　金：86 3计划资助项目!( 86 3 10 1 0 1 0 1 0 1;Z17 0 1 0 1)

摘　　要：应用基因枪法转化了小麦幼胚、成熟胚及胚性愈伤组织,成功地将携带有豇豆胰蛋白酶抑制剂(CpTI)基因以及选择标记潮霉素磷酸转移酶(Hpt)基因的质粒pBCAHpt导入小麦胚性愈伤组织。通过在选择培养基上严格筛选后获得潮霉素抗性(hygr)愈伤组织,并进一步分化培养获得完整的小麦再生植株。PCR检测结果显示抗性愈伤组织和再生植株细胞内存在有目的基因序列。Immature, mature wheat embryos and embryogenic calli were used as the plant recipient material for genetic transformation by high velocity microprojectile bombarment, Plasmid pBCA hpt containing insecticidal Cowpea Trypsin Inhibitor and Hpt gene was delivered into wheat cells. After strict selection, some hygromycin resistant calli were obtained, and plantlets regenerated from the calli on the differentiation medium. The PCR analysis indicated plants regenerated from resistant calli contained the target gene, and showed they were transgenic plants.

关 键 词：小麦转化 基因枪法 潮霉素抗体 转基因植株 

分 类 号：S512.1035.4[农业科学—作物学]