应用多重PCR法分析西藏察隅疟疾流行区按蚊吸血习性  

Investigation on Blood-sucking Habit of Anopheles(Diptera:Culicidae) Using Multiplex Polymerase Chain Reaction in Malaria-Endemic Area of Chayu County,Tibet

作　　者：郭绍华[1] 周水森[1] 黄芳[1] 郑香[1] 武松[2] 周华云[3] 卓玛央金 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025 [2]安徽中医学院中西医结合临床学院,合肥230038 [3]江苏省寄生虫病防治研究所,无锡214064 [4]西藏自治区林芝地区疾病预防控制中心,林芝860100

出　　处：《中国寄生虫学与寄生虫病杂志》2012年第2期122-126,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：中国/WHO 2010-2011年合作项目(No.CHN188-11);卫生行业科研专项项目(No.200802021)~~

摘　　要：目的用多重PCR法分析西藏疟疾流行区察隅县常见按蚊的吸血习性,为下一步研究传疟媒介提供参考。方法2011年7~8月选择察隅县不同生态环境的3个自然村（日玛村、塔玛村和京都村）,每个村在人房和畜舍选择8个点,采用诱蚊灯全通宵（20∶00至次日08∶00）诱捕法捕捉按蚊,次日清晨收集诱捕的蚊虫,经形态学鉴定蚊种,分析按蚊组成。收集饱血按蚊,分别提取单只蚊胃血的DNA,采用基于不同动物mtDNA-cytb序列差异的多重PCR法鉴定各蚊胃血源,计算人血指数,分析按蚊的吸血习性。结果共捕获按蚊1 442只,经形态学鉴定,多斑按蚊种团占99.6%（1 436/1 442）,带足按蚊和腹簇按蚊占0.4%（6/1442）。多斑按蚊种团中,伪威氏按蚊占85.5%（1228/1436）,威氏按蚊占14.5%（208/1 436）。用多重PCR检测202只多斑按蚊种团（伪威氏按蚊188只和威氏按蚊14只）的饱血蚊胃血,结果显示,伪威氏按蚊兼吸牛/猪血和人血,人血指数为0.35,威氏按蚊吸食猪血和人血,人血指数为0.29。结论西藏察隅县2种常见按蚊（伪威氏按蚊和威氏按蚊）均兼吸人畜血,伪威氏按蚊的人血指数较高。Objective To determine the blood-sucking habit of anopheline by mosquitoes bloodmeal identification in malaria endemic area Chayu County,Tibet Autonomous Region.Methods Three villages with different bio-enviroments were selected as the investigation spots.Light traps were set up outdoor and in livestock sheds from sunset（20 ∶ 00） to sunrise（8 ∶ 00） in 3-4 consecutive nights to collect mosquitoes.The trapped anophelines were counted and identified according to morphological criteria and multiple PCR method.A PCR-based methodology according to the mtDNA-cytb variations was used in different mammal hosts to identify bloodmeal sources in engorged mosquitoes.The human blood index（HBI） was assessed to determine the range of hosts.Results Among 1 442 anopheline mosquitoes collected by 108 lighttraps on 13 nights.1 436（99.6%） belonged to Anopheles maculatus complex,with 85.5% An.pseudowillmori and 14.5% An.willmori.Positive bloodmeal identification was found from 168（83%） of 202 field-collected engorged mosquitoes.The crude HBI of An.pseudowillmori and An.willmori were 0.35 and 0.29,respectively.Conclusion An.pseudowillmori and An.willmori are both zoophilic and anthropophilic,and An.pseudowillmori shows a higher HBI.

关 键 词：西藏察隅县 疟疾 按蚊 多重PCR 人血指数 

分 类 号：R384.111[医药卫生—医学寄生虫学]