弓形虫对4种肿瘤细胞增殖及凋亡的影响  

Impact of Toxoplasma gondii on the Proliferation and Apoptosis of Tumor Cell Lines

作　　者：武欣[1] 孙黎[2] 张力[3] 刘正泉[4] 罗强[2] 张林西[2] 

机构地区：[1]河北北方学院研究生部,张家口075000 [2]河北北方学院生命科学研究中心,张家口075000 [3]西安医学院基础医学院,西安710021 [4]河北北方学院成人教育学院,张家口075000

出　　处：《中国寄生虫学与寄生虫病杂志》2012年第2期157-159,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河北省张家口市科技局资助课题(No.0911021D-3)~~

摘　　要：用1×104个/ml^32×104个/ml 6个不同浓度刚地弓形虫RH株速殖子体外分别作用于对数生长期的乳腺癌MCF-7、前列腺癌DU-145、食管癌EC-109和肺癌A549细胞(5×104/ml)24 h,运用CCK-8法测定弓形虫对上述肿瘤细胞的抑制作用,计算细胞抑制率。用1×104、2×104和4×104个/ml弓形虫速殖子体外作用于A549细胞24 h后,流式细胞仪检测肿瘤抑制基因P53和B细胞淋巴瘤/白血病-2(Bcl-2)基因所编码蛋白质的表达情况。结果显示,随着弓形虫速殖子浓度升高,各肿瘤细胞增殖抑制率增高,其效果与剂量呈正相关。1×104个/ml弓形虫速殖子即对MCF-7、DU-145、EC-109和A549细胞增殖有抑制作用,其抑制率分别为19.7%、4.5%、28.0%和20.8%。各实验组肿瘤细胞增殖情况与对照组比较差异均有统计学意义(P<0.05)。流式细胞术检测A549细胞凋亡,凋亡率上升,且p53蛋白表达上调、Bcl-2蛋白表达下调。MCF-7,DU-145,EC-109 and A549 tumor cells（5×104/ml） at mid-exponential phase were treated for 24 h with different concentrations of Toxoplasma gondii tachyzoites（1×104/ml-32×104/ml）.The measurement of tumor cell growth inhibition rate was performed by using CCK-8 kit.The tachyzoites（1×104/ml-4×104/ml） were co-cultured with A549 tumor cells for 24 h.Flow cytometry techniques was applied to investigate the expression of p53 and Bcl-2.The results showed a remarkable inhibitory effect of the parasites on tumor cell proliferation,which presented a dosage dependence.MCF-7,DU-145,EC-109 and A549 cell lines treated with 1×104/ml T.gondii tachyzoites presented an inhibition rate of 19.7%,4.5%,28.0%,and 20.8%,respectively.There was a significant difference between experimental groups and the control（P0.05）.T.gondii induced cell apoptosis in A549 tumor cells.p53 and Bcl-2 gene expression was respectively up-regulated and down-regulated.

关 键 词：弓形虫 肿瘤细胞 流式细胞术 CCK-8法 

分 类 号：R382.5[医药卫生—医学寄生虫学]