淫羊藿总黄酮中乙酸乙酯、正丁醇溶剂残留量的测定  

Determination of Ethyl Acetate and N-butanol Residual Volume in Epimedium Total Flavonoids

作　　者：周蕾[1] 雷永涛[1] 郝小燕[1] 李谦[1] 梁妍[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第2期123-126,共4页Journal of Guiyang Medical College

基　　金：贵州省科技厅中药现代化科技产业研究开发专项项目[黔科合中药字(2010)5021号];贵阳市科学技术计划项目[(2008)筑科农合字第24-2号];贵阳市科学技术计划项目[(2010)筑科农合字第1-中-06号]

摘　　要：目的:建立气相色谱法测定淫羊藿总黄酮中有机溶剂乙酸乙酯和正丁醇残留量的方法。方法:采用毛细管气相色谱法,以SE-54毛细管柱为色谱柱,程序升温,氮气为载气,进样口温度为230℃,FID检测器温度为240℃。结果:乙酸乙酯和正丁醇残留量分别在1.606～16.06 mg/L(r=0.999 1)、1.622～16.22 mg/L(r=0.999 3)的浓度范围内呈良好的线性关系,三批样品中乙酸乙酯和正丁醇残留量均符合中国药典有机溶剂残留量规定。结论:毛细管气相色谱法可作为淫羊藿总黄酮提取物中有机溶剂残留量的测定方法。Objective： To establish a method for determination of the residual solvents including ethyl acetate and N-butanol in epimedium total flavonoids by gas chromatography（GC）.Methods： Gas Chromatography was carried out on temperature programmed SE-54 capillary column using nitrogen as carrier gas.The injection port temperature was 230 ℃.The temperature of FID detector was 240 ℃.Results： The calibration curves for Ethyl acetate and n-butanol were linear in the range of 1.606~16.06 mg/L（r=0.999 1）and 1.622~16.22 mg/L（r=0.999 3） respectively.The residual volume of ethyl acetate and N-butanol in three batches of samples accorded with Chinese Pharmacopoeia requirement of organic solvent residue.Conclusions： The method can be used for determination of organic solvent residual volume in epimedium flavonoids.

关 键 词：黄酮 淫羊藿 乙酸乙酯 正丁醇 药物残留物 色谱法 气相 

分 类 号：R927.1[医药卫生—药学]