淫羊藿总黄酮的提取和分离纯化工艺研究  

Study on Extraction and Purification Process of Total Flavonoids from Herba epimedii

作　　者：雷永涛[1] 李莉[1] 郝小燕[1] 周蕾[1] 梁妍[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第2期137-140,共4页Journal of Guiyang Medical College

基　　金：贵州省科技厅中药现代化科技产业研究开发专项项目[黔科合中药字(2010)5021];贵州省科学技术基金项目(合同号:20082153);贵阳市科学技术计划项目[黔科合中药字(2010)筑科农合同字1-中-06号]

摘　　要：目的:优选淫羊藿总黄酮的提取纯化工艺。方法:采用L9(34)正交设计法,以浸膏得率和总黄酮含量为检测指标优选提取工艺;用AB-8型大孔吸附树脂柱进行分离,乙酸乙酯、正丁醇萃取纯化。结果:淫羊藿总黄酮的最佳提取工艺为淫羊藿叶的粗粉中加入27倍量水(V/W),浸泡2 h后,煎煮3次,每次1 h;淫羊藿提取液上AB-8型大孔吸附树脂柱分离,再用乙酸乙酯、正丁醇萃取,所得淫羊藿总黄酮的平均含量(以淫羊藿苷计)为60.91%。结论:该工艺稳定可行,提取淫羊藿总黄酮的含量较高,可为工业生产提供依据。Objective： To optimize extraction and purification process of total flavonoids from Herba Epimedii.Methods： The optimum extractive technology was investigated by using L9（34） design with the extracting rate and the contents of total flavonoids as evaluation indexes.AB-8 macroporous resign column was used to isolate flavonoids,and ethyl acetate,N-butanol was used to extract and purify flavonoids.Results： The optimum extraction condition was： adding 27 times amount of water to Herba Epimedii leaf powder,soaking 2h,boiling 3 times each time for 1h,loading epimedium extracts into AB-8 macroporous resign column,and separating with ethyl acetate and N-butanol.The average content of total flavonoids from epimedium（in terms of icariin） was 60.91%.Conclusion： This process is stable,feasible,and the extraction of epimedium flavonoids contents is higher,which may provide reference for industrial production.

关 键 词：淫羊藿 黄酮 中草药 制药工艺 

分 类 号：R285.5[医药卫生—中药学]