过量表达甜菜BvNHX1基因提高拟南芥的耐盐性  

OVEREXPRESSION OF BVNHX1 FROM BETA VULGARIS TO IMPROVE SALT TOLERANCE IN ARABIDOPSIS THALIANA

作　　者：王鹏[1] 安静[2,1] 侯蕾[2] 孔祥强[2,1] 赵彦修[2] 张慧[2] 

机构地区：[1]山东省农业科学院,山东济南250100 [2]山东师范大学、山东省逆境植物重点实验室,山东济南250014

出　　处：《山东农业大学学报（自然科学版）》2012年第2期163-168,178,共7页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：Na+/H+逆向转运蛋白调节细胞内的离子平衡,在植物耐盐性起重要的作用。为了研究甜菜液泡膜Na+/H+逆向转运蛋白BvNHX1基因在植物耐盐中的作用,构建了植物表达载体pROKⅡ-BvNHX1转化拟南芥.在含有卡纳霉素的培养基上筛选转化子,并利用Southern和Northern杂交技术检测,进一步证实BvNHX1基因已整合到拟南芥基因组并能正常转录。选取纯合转基因株系进行耐盐性分析实验表明,过量表达BvN-HX1基因的拟南芥在种子萌发和苗期都提高了植株耐盐性,盐处理下转基因植株的鲜重、干重以及地上部分Na+、K+含量均高于野生型对照。结果表明过量表达BvNHX1基因提高了转基因拟南芥的耐盐能力。Na＋/H＋ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis.In order to reveal the function of BvNHX1 from Beta vulgaris in plant salt resistance,the plant expression vector pROKⅡ-BvNHX1 was constructed and introduced into Arabidopsis thaliana.Transformants were selected for their ability to grow on a medium containing kanamycin.Southern blot analyses confirmed that BvNHX1 was transferred into the Arabidopsis thaliana genome and northern blot analyses showed that BvNHX1 was expressed in the transformants.Several transgenic homozygous lines and wild-type plants were evaluated for salt tolerance,the results suggest that transgenic Arabidopsis thaliana plants expressing BvNHX1 improved seed germination rate and salt tolerance in seedlings.Under saline conditions,both fresh and dry weights of the transgenics as well as their accumulations of Na＋ and K＋ were much higher than that of wild-type.These results demonstrate that overexpression of BvNHX1 in Arabidopsis thaliana increased salt tolerance of transgenic plants compared with the wild-type plants.

关 键 词：盐生植物 BvNHX1 液泡膜Na+/H+逆向转运蛋白 耐盐性 拟南芥 

分 类 号：Q94[生物学—植物学]