不同针刺方法对慢性应激抑郁大鼠下丘脑CRH基因表达及血清ACTH、CORT的影响  

Influences of different acupuncture methods on hypothalamic CRH mRNA expression and serum ACTH and CORT in rats with chronic stress depression

作　　者：梁佳[1] 卢峻[1] 王俊仁[1] 崔善福[1] 阿英格[2] 图娅[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]内蒙古医学院基础医学院

出　　处：《北京中医药大学学报》2012年第4期265-268,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81173334),国家自然科学基金青年基金项目(No.81102638);北京市优秀博士学位论文指导教师科技项目(No.YB20091002601);北京中医药大学自主课题项目(No.2009JYBZZ-X5050,No.2011JYBZZ-JS067)

摘　　要：目的对比观察手针与电针对慢性应激抑郁模型大鼠下丘脑促肾上腺皮质激素释放激素(CRH)mRNA表达及血清促肾上腺皮质激素(ACTH)、皮质酮(CORT)影响的差异,探讨手针与电针干预治疗抑郁症的机制。方法将SD大鼠随机分为4组:空白组、模型组、手针组、电针组,采用实时荧光定量PCR(RT-PCR)方法检测下丘脑CRH mRNA含量,酶联免疫吸附法(ELISA)检测大鼠血清ACTH、CORT含量。结果与空白组相比,模型组大鼠下丘脑CRH mRNA的含量显著升高(P<0.01),血清CORT和ACTH含量显著升高(P<0.01)。与模型组相比,手针组与电针组大鼠下丘脑CRH mRNA的含量均明显降低(P<0.05、P<0.01);手针组与电针组大鼠血清CORT表达有所下降,但无统计学差异(P>0.05);手针组与电针组大鼠血清ACTH表达显著降低(P<0.05、P<0.01)。结论慢性应激抑郁模型大鼠表现出HPA轴功能亢进,手针和电针均可以下调HPA轴功能,拮抗HPA轴功能亢进可能是手针和电针发挥抗抑郁治疗作用的途径之一。Objective To compare and observe the differences between the influences of acupuncture and electroacupuncture on the expression of hypothalamic corticotrophin-releasing hormone（CRH） mRNA and content of serum adrenocorticotropic hormone（ACTH） and corticosterone（CORT） in rat model of chronic stress depression,and investigate the mechanism of acupuncture and electroacupuncture in intervene and treatment of depression.Methods All SD rats were randomly divided into blank group,model group,acupuncture group and electroacupuncture group.The expression of hypothalamic CRH mRNA was detected by using real-time fluorescence quantitative polymerase chain reaction（RT-PCR）,and content of serum ACTH and CORT were detected by applying enzyme-linked immunosorbent assay（ELISA）.Results Compared with blank group,the expression of CRH mRNA and content of serum ACTH and CORT increased significantly in model group（P0.01,P0.01）.Compared with model group,the expression of CRH mRNA decreased significantly in acupuncture group and electroacupuncture group（P0.05、P0.01）.The content of serum CORT decreased but had no statistical difference（P0.05）,and ACTH expression decreased significantly（P0.05、P0.01） in acupuncture group and electroacupuncture group.The differences of above indexes were not significant between acupuncture group and electroacupuncture group（P0.05）.Conclusion The rat model of chronic stress depression had a hyperactivity of HPA axis.Acupuncture and electroacupuncture can down-regulate the function of HPA axis.It may be one of ways to treat depression with acupuncture and electroacupuncture that they have antagonism to the hyperactivity of HPA axis.

关 键 词：手针 电针 慢性应激 HPA轴 抑郁症 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]