龙竹和麻竹FT同源序列的扩增与序列分析  

Amplification and Sequence Analysis of Partial FT-like Sequences in Dendrocalamus giganteus and Dendrocalamus latiflorus

作　　者：桑庆亮[1] 黄周英[1] 孙亮先[1] 

机构地区：[1]泉州师范学院化学与生命科学学院,福建泉州362000

出　　处：《泉州师范学院学报》2012年第2期67-71,共5页Journal of Quanzhou Normal University

基　　金：福建省科技厅青年人才项目(2007F3087);泉州市科技局计划项目(2008Z15)

摘　　要：根据拟南芥Flowering Locus T(FT)基因和水稻Heading date 3a(Hd3a)基因的序列设计引物,通过PCR扩增的方法分别从麻竹和龙竹基因组DNA中各得到长为334bp的DNA片段.经BLAST检索、基因结构分析和编码蛋白功能域预测等分析,初步确定所得DNA片段可能是麻竹和龙竹中的FT同源基因的部分序列.In this paper,primers were designed according to the conserved exons of Arabidopsis thaliana Flowering Locus T（FT） gene and Oryza sativa Heading date 3a（Hd3a） gene to amplify the corresponding DNA sequence from genomic DNA of D.latiflorus and D.giganteus,and two 334 bp long DNA sequences were obtained.Through blast search,gene structure analysis and conserved domain search,we conclud that these two DNA sequences might be members of FT gene family in D.latiflorus and D.giganteus.

关 键 词：麻竹 龙竹 FT同源基因 PCR扩增 序列分析 

分 类 号：Q523[生物学—生物化学] S718.46[农业科学—林学]