苯酚-硫酸法测定保健酒药渣中总多糖的含量  

The Determination of Total Polysaccharides from the Herb Residue of Health Wine with Phenol-sulfuric Acid Method

作　　者：罗海燕[1] 冯棋琴 马小花 徐霞 周海妹 索昀娜 

机构地区：[1]海南医学院,海口5711011 [2]海南椰岛(集团)股份有限公司,海口570105

出　　处：《食品与发酵科技》2012年第2期97-100,共4页Food and Fermentation Science & Technology

基　　金：海南省重点科技计划项目(090140)

摘　　要：通过水提醇沉法提取保健酒药渣中总多糖,采用苯酚-硫酸法测定总多糖的含量。结果表明,药渣提取液与葡萄糖标准液在波长490nm处均有最大吸收,葡萄糖标准液在2.277～11.387μg/mL范围内与吸光度值有良好的线性关系,苯酚-硫酸法测定药渣总多糖其精密度、稳定性、重复性以及加样回收率均良好,测得保健酒药渣中总多糖(以葡萄糖计,下同)含量为5.62%。The total polysaccharides were obtained from the herb residue of health wine with water extraction and alcohol precipitation method,and the polysaccharides content of the extraction was measured with the phenol-sulfuric acid method.The results showed that ： the herb residue extract and standard glucose solution all had absorption maximum at 490nm;there was a good linearity relation between the absorbency and the content of standard glucose in the range of 2.277-11.387μg/mL.In addition,the Phenol-sulfuric acid method had good accuracy,stability,repeatability and recovery.By this method,the average content of total polysaccharides（glucose） in herb residue of health wine was 5.62%.

关 键 词：药渣 总多糖 苯酚-硫酸法 

分 类 号：TS261.7[轻工技术与工程—发酵工程]