板栗苞单宁纯化的研究  

Study on Purification of Chestnut Shell Tannin

作　　者：杜运平[1] 秦清[1] 徐浩[1] 张宗和 黄嘉玲 陈文文[1] 陶林 

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,江苏南京210042 [2]南京龙源天然多酚合成厂,江苏南京210032

出　　处：《林产化学与工业》2012年第2期61-65,共5页Chemistry and Industry of Forest Products

基　　金：国家林业局林业公益性行业科研专项(201004013)

摘　　要：以通常废弃的板栗苞为原料,经过提取得到板栗苞单宁提取物,并对提取物的纯化进行了研究。分别研究了吸附剂的选择、柱前液质量浓度、洗脱剂、蒸馏水的pH值、流速等单因素对单宁得率的影响,并通过正交试验得出了纯化的最佳工艺条件,即:选用大孔树脂D301为吸附剂,丙酮为洗脱液,柱前液质量分数为9%,蒸馏水pH值为7,过柱流速为0.4 L/h。最后,通过稳定性实验对所得工艺条件进行了验证,按此工艺条件纯化后的板栗苞单宁得率在82%以上,单宁质量分数在62%以上。Purification of chestnut shell tannin from generally abandoned chestnut shell was studied.The effect of adsorbent,pre-column concentration,eluent,pH value of distilled water and flow rate on tannin yield was tested respectively.According to single factor and orthogonal experiment results,the optium experimental conditions were as follows： macroporous resin D301 as adsorbent,acetone as eluent,pre-column concentration 9%,pH value 7.0 of distilled water,flow rate 0.4 L/h.Finally,the best process condition was verified by stability test.Under these conditions,the yield of chestnut shell tannin was up to 82%,and tannin mass fraction was higher than 62%.

关 键 词：板栗苞 单宁 纯化 大孔树脂 

分 类 号：TQ35[化学工程]