口蹄疫病毒Mya98谱系猪源及牛源2010年分离株的分子特征及蚀斑显型比较分析  

Molecular characteristics and plaque phenotype of Mya98/FMDV isolated from different hosts in 2010

作　　者：周强[1] 包慧芳[1] 白兴文[1] 孙普[1] 陈应理[1] 谢宝霞[1] 卢曾军[1] 刘在新[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室甘肃省生物检测工程技术研究中心国家口蹄疫参考实验室OIE口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2012年第4期331-338,共8页Chinese Veterinary Science

基　　金：农业行业专项(201103008);国家高技术研究发展计划(863)项目(2011AA10A211);兰州市科技计划项目(2010-1-175)

摘　　要：为了明确口蹄疫病毒(FMDV)Mya98谱系猪源及牛源2010年分离株的分子特征及蚀斑显型差异,对采自不同宿主的4株FMDV进行了分子特征分析和蚀斑形态的比较。结果显示,来自不同宿主动物的分子特征差异主要表现为猪源毒株O/CHN/Mya98/2010S2与2个牛源毒株之间具有21个特异性的氨基酸置换位点,分散在ORF范围内。猪源毒株O/CHN/Mya98/2010S1具有与牛源毒株大量相似的氨基酸位点,并且与2株牛源毒株一样,都显示为小斑的蚀斑显型,而猪源毒株O/CHN/Mya98/2010S2在蚀斑试验中显示为大小斑混合存在。结果表明,猪源毒株O/CHN/Mya98/2010S2的变异位点对病毒蚀斑显型的改变具有重要意义。For understanding the molecular characteristics and plaque phenotype of Mya98/FMDV isolated from different hosts in 2010,molecular characteristic analysis and plaque phenotype comparison of 4 foot-and-mouth disease viruses（FMDV） isolated from various hosts were performed.And the result revealed that the isolates were of the swine strain O/CHN/Mya98/2010S2.This strain shared 21 specific amino acid substitution（AAS） sites with bovine strains,which were distributed within the range of the open reading frame（ORF）.The swine strain O/CHN/Mya98/2010S1 also shared a number of similar amino acid sites with bovine strains,and both of the identified strains demonstrated small plaque phenotype.However,swine strain O/CHN/Mya98/2010S2 manifested as a mixture of small and large plaques in the plaque assay,suggesting that the variable site of this strain was of significance to the change in virus plaque phenotype.

关 键 词：O型口蹄疫病毒 Mya98谱系 不同宿主 分子特征 蚀斑显型 

分 类 号：S852.659.6[农业科学—基础兽医学]