旋毛虫感染小鼠后TNF-α诱导心肌细胞凋亡的试验观察  

Experimental observation on TNF-α induced myocardial apoptosis in myocardium of the mice infected with Trichinella spiralis

作　　者：韩彩霞[1] 路义鑫[1] 李晓云[1] 张子群 赵风强[1] 宋铭忻[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]黑龙江出入境检验检疫局,黑龙江哈尔滨150001

出　　处：《中国兽医科学》2012年第4期357-361,共5页Chinese Veterinary Science

基　　金："十一五"国家科技支撑计划重大项目(2010BAD04B01);家畜疫病病原生物学国家重点实验室开放课题(SKLVEB2009KFKT005);博士后研究人员落户黑龙江科研启动资助基金项目(LBH-Q10150)

摘　　要：为探讨旋毛虫致小鼠心肌损伤发生的主要致病因子及其信号传导途径,分别取感染前后不同时期小鼠心肌,应用半定量RT-PCR法检测心肌中炎性细胞因子TNF-α及细胞凋亡与抗凋亡基因mRNA表达量,并应用TUNEL法对心肌细胞凋亡形态进行了检测。结果显示,旋毛虫感染后第9天,TNF-αmRNA的相对表达量由感染前的0.02迅速增加到0.88,心肌细胞凋亡增加,凋亡指数为10.70%;TNF-α表达量约于感染后第19～24天达到峰值,此时凋亡指数达29.79%,细胞凋亡明显(P<0.05);第42天TNF-α表达量逐渐下降至0.29,细胞凋亡逐渐减少,凋亡指数降至10.46%。TNFR 1、FADD、Caspase 8和Caspase 3mRNA的相对表达量的变化趋势与TNF-α相似。To explore the main pathogenic agent and its signal transduction of myocardial damage caused by Trichinella spiralis infection,mRNA of TNF-α,related apoptotic,and antiapoptotic genes in myocardium were determined using semi-quantitative RT-PCR and cell apoptosis was investigated using TUNEL assay at different time during Trichinella spiralis infection of the mice.Results showed that the relative expression level of TNF-α was from 0.02（before infection） to 0.88（on day 9 post-infection）,and reached peak from days 19 to 24 post-infection,then decreased to 0.29 on day 42 post-infection.Apoptosis index,meanwhile,increased from 10.70% to 29.79%（P0.05）,and then fell to 10.46%.The mRNA expressions of TNFR 1,FADD,Caspase 8 and Caspase 3 showed a similar tendency.

关 键 词：旋毛虫 TNF-Α 细胞凋亡 心肌损伤 

分 类 号：S852.731[农业科学—基础兽医学]