三株鸭源H9N2亚型禽流感病毒福建株全基因的测序及遗传进化分析  

Characterization of the complete genome of three strains of duck-origin H9N2 subtype avian influenza virus isolated from Fujian

作　　者：万春和 傅光华 施少华 程龙飞 陈红梅 黄瑜 

机构地区：[1]福建农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心,福建福州350013

出　　处：《中国兽医科学》2012年第4期362-367,共6页Chinese Veterinary Science

基　　金：现代农业产业体系建设专项(CARS-43);国家科技支撑项目(2006BAD06A01);福建省种业创新与产业化工程项目(FJZYCX-9);福建省重点项目(2009N4001);科技人员服务企业行动项目(2009GJC40037);福建省科技厅星火计划项目(2009S0110)

摘　　要：为明确3株鸭源H9N2亚型禽流感病毒福建株A/Muscovy duck/Fujian/CL/1997(H9N2)(简称CL株)、A/duck/Fujian/MH/2003(H9N2)(简称MH株)和A/duck/Fujian/FQ2/2007(H9N2)(简称FQ2株)全基因组的分子特征和遗传进化情况,采用RT-PCR方法扩增这3株病毒全基因片段,并进行序列比较及遗传进化分析。结果表明,所克隆的8个基因片段均有完整的开放阅读框,其血凝素基因蛋白裂解位点的氨基酸组成为-RSSR*GLF-,为不连续的碱性氨基酸,其静脉接种指数远小于1.2,均符合低致病性AIVHA蛋白裂解位点特征;CL株和MH株的HA基因在遗传进化上属于常见的CK/BJ/1/94群系;FQ2株HA基因在遗传进化上属于DK/HK/Y280/97群系,3株H9N2亚型AIV HA基因在遗传进化上均属于经典的欧亚种系。3株H9N2亚型AIV NA基因颈部均存在缺失,CL株和MH株的NA基因在遗传进化上属于常见的CK/BJ/1/94群系,FQ2株NA基因在遗传进化上属于G1群系。CL株和MH株的其他6个基因在遗传进化上均和经典的CK/BJ/1/94一致,而FQ2株则可能是由不同流感病毒基因亚系间发生自然重排的产物。To analyse the genetic characteristics of duck-origin H9N2 subtype avian influenza virus（AIV）,the full genome of three strains,namely H9N2 subtype AIV＇s [A/Muscovy duck/Fujian/CL/1997（H9N2）、A/duck/Fujian/MH/2003（H9N2） and A/duck/Fujian/FQ2/2007（H9N2）] was amplified by reverse transcription-polymerase chain reaction（RT-PCR）,and then sequenced and phylogeneticly analyzed.The results demonstrated that the hemagglutinin genes＇ deduced amino acid sequence at the cleavage site of the HA protein were-RSSR＊GLF-,and the IVPI index were all far below 1.2,which matched the characteristics of low pathogenic avian influenza（LPAI）.The CL and the MH strains belonged to the CK/BJ/1/94 sublineage,while the FQ2 strain belonged to Y280 sublineage,all were on the classical Euro-Asia lineage.The neuraminidase gene had deletions at 63,64 and 65 amino acids,the CL and MH strains belonged to the CK/BJ/1/94 sublineage,while the FQ2 strain belonged to G1 sublineage.The CL andMH strains were phylogeneticly closely related to the classical CK/BJ/1/94 sublineage based on sequences of the other six genes,while the FQ2 strain might be the product of natural reassortment from different influenza viruses sublineages.

关 键 词：鸭源H9N2亚型禽流感病毒 全基因 序列分析 遗传进化 

分 类 号：S852.659.5[农业科学—基础兽医学]