蜂毒素对人肝癌细胞中HMGB1及VEGF-C表达的影响  

Effect of melittin on high mobility group box 1 and vascular endothelial growth factors C expressions in hepatocarcinoma cells

作　　者：曹清心[1,2] 汪晨[1] 刘宇[3] 赵丹[4] 凌昌全[1] 

机构地区：[1]第二军医大学长海医院中医科,上海200433 [2]解放军455医院,上海200052 [3]第二军医大学长海医院心血管内科,上海200433 [4]第二军医大学长海医院呼吸内科,上海200433

出　　处：《东南国防医药》2012年第2期101-104,共4页Military Medical Journal of Southeast China

基　　金：国家自然科学基金资助项目(30901986);上海市晨光计划资助项目(10CG41);南京军区医学科技创新课题项目(10MA028)

摘　　要：目的观察蜂毒素对人肝癌细胞株HepG2中高迁移率族蛋白B1(high mobility group box1,HMGB1)及血管内皮生长因子C(vascular endothelial growth factors C,VEGF-C)表达的影响,探讨其抑制肝癌细胞的作用机制。方法肝癌细胞HepG2体外培养,经蜂毒素处理后,采用四甲基偶氮唑蓝(MTT)法了解蜂毒素对肝癌细胞增殖的影响,用Western-blotting、qRT-PCR方法检测HMGB1、VEGF-C的表达。结果蜂毒素在体外能够抑制肝癌细胞的增殖活性;Western-blotting结果显示,蜂毒素可抑制HMGB1的表达,且呈浓度依赖性,但对VEGF-C的蛋白表达无明显影响;qRT-PCR结果显示,蜂毒素在mRNA水平均具有抑制HMGB1、VEGF-C表达的作用。结论蜂毒素可降低肝癌细胞的增殖活性,并可能通过HMGB1、VEGF-C的表达发挥抗肝癌作用。Objective To observe the effect of melittin on the expressions of high mobility group box 1（HMGB1） and vascular endothelial growth factors C（VEGF-C） in hepatocarcinoma cells in vitro and to study the mechanisms of melittin in hepatocarcinoma cells.Methods HepG2 cell line was treated with melittin in vitro.The inhibition of proliferation was detected by MTT assay.The expressions of HMGB1 and VEGF-C were detected by western-blotting and real-time quantitative PCR（qRT-PCR） assay.Results Melittin inhibited cell proliferation in vitro.Western-blotting outcome showed that melittin could down-regulate the protein of HMGB1,while VEGF-C expression did not change.qRT-PCR results showed that HMGB1 and VEGF-C mRNA expressions were down-regulate by melittin.Conclusion It is suggested that melittin can inhibit hepatocarcinoma cells proliferation.The effect of melittin in inducing anti-hepatocarcinoma may be related with down-regulation of HMGB1 and VEGF-C.

关 键 词：蜂毒素 肝细胞癌 高迁移率族蛋白B1 血管内皮生长因子C 

分 类 号：R735.7[医药卫生—肿瘤]