芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin 1的作用  

Influence of Fentanyl on Expression of δ Receptor and β-arrestin 1 in Locus Ceruleus of Chronic Morphine Tolerant Rats

作　　者：刘若杉[1] 孙莉[1] 

机构地区：[1]中国医学科学院肿瘤医院麻醉科,北京市100021

出　　处：《中国康复理论与实践》2012年第4期333-336,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：首都医学发展科研基金项目(20052057)

摘　　要：目的研究芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin 1的作用。方法 40只成年雄性SD大鼠随机分为5组,每组8只。NS组:注射生理盐水1 ml/kg 2次;M组:分别注射吗啡10 mg/kg与生理盐水1 ml/kg;MF1、MF2、MF3组:吗啡用药同M组,30 min后分别给予芬太尼3、6、12μg/kg,连续9 d。取大鼠蓝斑核,测定δ受体、β-arrestin 1的mRNA与蛋白表达。结果与NS组比较,M组δ受体、β-arrestin 1表达明显下调(P<0.01);与M组比较,MF1、MF2和MF3组δ受体表达无显著性差异(P>0.05),但MF2组和MF3组β-arrestin 1 mRNA及其蛋白的表达增加(P<0.05)。结论芬太尼6μg/kg、12μg/kg可上调慢性吗啡耐受大鼠蓝斑核β-arrestin 1表达,但对δ受体表达无影响。Objective To explore the effects of fentanyl on expression of δ receptor and β-arrestin 1 in locus ceruleus of chronic morphine tolerant rats.Methods 40 male SD rats were randomly allocated to 5 groups with 8 cases in each group.Group NS received only normal saline 1 ml/kg twice;Group M received morphine 10 mg/kg followed by normal saline 1 ml/kg;Groups MF1,MF2 and MF3 received morphine 10 mg/kg followed by fentanyl 3,6,12 μg/kg respectively.All animals were killed on the 9th day after measurement of pain threshold.Locus ceruleus was removed for determination of the expression of mRNA（RT-PCR） and protein（Western blotting） of δ receptor and β-arrestin 1.Results The expression of δ receptor and β-arrestin 1 was significantly decreased in Group M than in Group NS（P0.01）.There was no significant difference in the expression of δ receptor mRNA and protein in Groups MF1,MF2 and MF3,and Group M（P0.05）.The expression of β-arrestin 1 mRNA and protein in Groups MF2 and MF3 significantly increased（P0.05）.Conclusion Fentayl with the dose of 6 and 12 μg/kg can dose-dependently increase the expression of β-arrestin 1 but δ receptor in locus ceruleus of chronic morphine tolerant rats.

关 键 词：吗啡耐受 芬太尼 蓝斑核 Δ受体 β-arrestin1 

分 类 号：R641[医药卫生—外科学]