当归补血汤对局灶性脑缺血再灌注损伤后大鼠神经功能影响  

作　　者：周凌云[1] 罗亚非[2] 娄金波[1] 胡先觉[1] 项永晶[1] 

机构地区：[1]贵阳中医学院第二附属医院,贵州贵阳550003 [2]贵阳中医学院解剖学教研室,贵州贵阳550002

出　　处：《实用中医药杂志》2012年第4期292-293,共2页Journal of Practical Traditional Chinese Medicine

摘　　要：目的:探讨当归补血汤对大鼠局灶性脑缺血再灌注损伤的保护作用。方法:雄性SD大鼠36只随机分为正常组、模型组、治疗组各12只。模型组和治疗组采用线栓法制备大鼠右侧大脑中动脉栓塞缺血再灌注模型。治疗组术后连续15天以0.36g/mL浓度当归补血汤3.6g/kg灌胃,每日2次(早、晚各1次)。治疗10天后进行各组大鼠神经行为学测试,用Nissl染色法观察大鼠海马CA1区神经元的形态和数量。结果:与模型组比较,治疗组神经功能评分增高,海马CA1区神经元数量增加(P<0.05)。结论:当归补血汤可改善脑缺血再灌注损伤大鼠的神经功能,减少大鼠海马CA1区神经元的死亡,对海马CA1区神经元具有保护作用。Objective：To investigate the protective effect of the decoction of angelicae sinensis for enriching blood on focal cerebral ischemia-reperfusion injury with rats.Methods：36 male SD rats will be randomLy divided into normal group,cerebral ischemia-reperfusion group（model group） and the decoction of angelicae sinensis for enriching blood-treatment group,12 in each group.A rat model of the right middle cerebral artery occlusion/reperfusion（MCAO） was created using filament method.Before being operated,treatment-group rats will be lavaged wish 0.36 g/mL the decoction of angelicae sinensis for enriching blood 3.6g/kg twice a day for 15 days（once in the morning and once in the evening）.10 days after operated,rats in each group of neurobehavioral tests,the numbers and morphological change of neurons in the Hippocampal CA1 will be observed through Nissl staining.Results：Compared with ischemia-reperfusion group,neurological function score increased in the decoction of angelicae sinensis for enriching blood treatment group.The number of neurons of the the Hippocampal CA1 was increased（P0.05）.Conclusion：Behavioral capabilities of the focal cerebral ischemia-reperfusion injury neurological function with rats were improved by the decoction of angelicae sinensis for enriching blood,the death of neurons in the Hippocampal CA1 was alleviated,neurons of Hippocampal CA1 was protected.

关 键 词：局灶性脑缺血再灌注 当归补血汤 神经功能评分 海马CA1区 神经元 

分 类 号：R256.491.262[医药卫生—中医内科学]