小鼠艾灸血清诱导肿瘤细胞凋亡的实验研究  

Experimental Study on Murine Moxibustion Serum Inducing Apoptosis of Tumor Cells

作　　者：陈云飞[1] 赵粹英[1] 陈汉平[1] 秦慧莲[2] 方舫[2] 

机构地区：[1]上海市针灸经络研究所,上海200030 [2]上海医科大学,上海200032

出　　处：《针刺研究》2000年第1期38-42,共5页Acupuncture Research

基　　金：国家自然科学基金!(No.395 70 882 )

摘　　要：运用艾灸血清和小鼠胸腺细胞瘤 (EL 4)进行细胞培养 ,观察艾灸血清在体外对肿瘤细胞形态、细胞周期及DNA断点的影响。结果发现艾灸血清能诱导肿瘤细胞凋亡 ,引起肿瘤细胞在形态上发生较典型的凋亡改变 ,影响肿瘤细胞由S期向G2 M期的转变过程 ,提高肿瘤细胞DNA断点标记阳性率。By culture EL 4 tumor cells with Murine Moxibustion Serum(MS),influence of MS to cell structure, cell cycle and breaking point of DNA was studied. The results proved that MS could induce apoptosis of tumor cells, result in typical apoptosis changes in vitro,influence transforming process of tumor cells from phase S to phase G 2M and enhance the positive marking percentage of breaking point of DNA. The conclusion is that MS may contain certian known or unknown ingredients which inhabit the growth of tumor cells.

关 键 词：肿瘤细胞培养 艾灸血清 细胞形态 细胞凋亡 

分 类 号：R245.81[医药卫生—针灸推拿学]