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作 者:林颖[1,2] 龙自立[1] 张璐[1] 叶庆富[1] 刘永立[1]
机构地区:[1]浙江大学农业与生物技术学院,浙江杭州310029 [2]浙江省玉环县科学技术局,浙江玉环317600
出 处:《核农学报》2012年第2期257-261,310,共6页Journal of Nuclear Agricultural Sciences
基 金:浙江省自然科学基金项目(Y3080202)
摘 要:以猕猴桃(Actindia chinensis)金桃品种的胚乳为外植体,探讨了培养基、生长调节剂和蔗糖浓度以及暗培养等条件对胚乳培养器官形成的影响。结果表明:2,4-D对猕猴桃胚乳愈伤组织的诱导效果显著;在20g/L的蔗糖浓度条件下,胚乳愈伤组织的器官分化效果最好;暗培养可以促进胚乳愈伤组织的生长和器官形成,其中暗培养7d效果最好。A regeneration protocol for cultured endosperm of Actinidia chinensis were set up via organogenesis from callus tissues derived from endosperm explants of Actinidia chinensis ' Jintao' in vitro. The effects of different media, plant growth regulators, sucrose concentrations and dark treatment on callus induction and shoot formation were studied. The addition of 2,4-D significantly increased percentage of calli formation form the endosperm explants. Addition of 20g/L of sucrose resulted in better organogenesis form, endosperm callus. Dark culture could promote the growth of endosperm callus and organ formation, and the best efficiency was obtained after 7d of culture in the dark.
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