不同产地蟾皮药材的HPLC指纹图谱研究  

Study on the HPLC Fingerprint of Toad Skin from Different Regions

作　　者：段林瑞[1] 张笑恺[1] 曹蔚[1] 谢艳华[1] 王四旺[1] 

机构地区：[1]第四军医大学药学系天然药物学教研室,陕西西安710032

出　　处：《中药材》2012年第2期182-187,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:对10批不同来源的蟾皮药材进行比较分析,建立蟾皮药材的指纹图谱,为其质量控制和药材鉴别提供依据。方法:采用高效液相色谱法进行检测,通过计算机辅助相似性评价系统对HPLC指纹图谱进行处理和分析,结合相似度计算对药材进行比较。结果:①建立了蟾皮药材HPLC指纹图谱共有模式,确定了29个峰构成蟾皮药材的指纹特征,其中9个吸收峰与已知9种蟾毒配基类标准品相对应;②10个不同来源的蟾皮药材相对保留时间相似度较高,均在0.99以上,但其相对峰面积相似度较低。结论:①该方法准确可靠、重复性好,其指纹图谱可用于蟾皮的鉴别和质量控制;②不同产地蟾皮药材在主要成分的构成上基本稳定,但各成分的含量却不尽相同。Objective：To study the HPLC fingerprint of toad skin and provide a reliable method for quality control and identification.Methods：It used HPLC for detection and Computer Aided Similarity Evaluation System for processing and analysing HPLC fingerprint.Results：①The common pattern of HPLC fingerprint of toad skin was astablished,29 peaks were identified as the characteristic fingerprints,in which 9 peaks corresponded to 9 bufogenins.②Each sample′s similarity of relative retention time was all above 0.99,but the similarity of relative peak areas was low.Conclusion：①The method is accurate and with good reproducibility.The fingerprints can be used for the identification and quality control of toad skin.②The toad skin from different regions are stable in composition,but the contents of the components are different.

关 键 词：蟾皮 不同产地 指纹图谱 高效液相色谱法 

分 类 号：R282.5[医药卫生—中药学]