北沙参药材的薄层色谱指纹图谱研究  

Fingerprint Analysis of Glehniae Radix by TLC

作　　者：苏星[1] 李相坤[2] 吴弢[2] 张彤[3] 王峥涛[1,2] 

机构地区：[1]中国药科大学生药学教研室,江苏南京210009 [2]上海中医药大学中药研究所/中药标准化教育部重点实验室,上海201203 [3]上海中医药大学教学实验中心,上海201203

出　　处：《中药材》2012年第2期210-213,共4页Journal of Chinese Medicinal Materials

基　　金：上海市教委曙光计划(09SG42);上海市科委启明星跟踪计划(11QH1402300)

摘　　要：目的:建立北沙参的薄层色谱指纹图谱,用于对北沙参药材进行真伪鉴别及质量控制。方法:以法卡林二醇、东莨菪素、伞形花内酯、异欧前胡素为对照品,采用薄层色谱法对10批不同采集地的北沙参商品药材及3批山东莱阳北沙参药材进行定性鉴别。采用双波长扫描法,以260 nm为参比波长,300 nm为检测波长,荧光模式进行扫描,得出北沙参的薄层扫描轮廓图。结果:由轮廓扫描图发现13批北沙参药材有8个主要峰,其中4个为法卡林二醇、东莨菪素、伞形花内酯、异欧前胡素,并且山东莱阳产北沙参中4个化合物的含量均高于其他10批商品药材。结论:该方法稳定、简便可行,可用于北沙参的真伪鉴别及质量控制。Objective： To establish TLC fingerprint of Glehniae Radix for the identification and quality control of the drug.Methods：10 batches of Glehniae Radix commercial drugs collected from different gathering areas and 3 batches from Laiyang,Shandong were used as qualitative identification samples.Falcarindiol、scopoletin、umbelliferone and isoimperatorin were used as the chemical reference substances.Double wavelength TLCS was performed with petroleum and ethyl acetate（4∶1） as developer,detection wavelength at 300 nm and reference wavelength at 260 nm.Results：TLC chromatogram of 13 samples had 8 well-resolved characteristic peaks,in which 4 peaks were falcarindiol,umbelliferone,scopoletin and isoimperatorin,respectively.Conclusion： The method is accurate and simple,and can be used for the quality control of Glehniae Radix.

关 键 词：北沙参 薄层色谱指纹图谱 法卡林二醇 东莨菪素 伞形花内酯 异欧前胡素 

分 类 号：R282.5[医药卫生—中药学]