Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation  

作　　者：崔玉琳 姜鹏 王金锋 李富超 陈英杰 郑国庭 秦松 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences [3]Key Laboratory of Marine Biotechnology,Ningbo University

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第3期471-475,共5页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China (973 Program) (No. 2011CB200902);the National High Technology Research and Development Program of China (863 Program) (Nos. 2009AA10Z106,2009AA064401);the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. 2010BSB02009);the National Natural Science Foundation of China (Nos. 40876082,30870247);the Science and Technology Project of Qingdao City (No.09-1-3-59-jch)

摘　　要：Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga.Platymonas （Tetraselmis） subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome ofP. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation ofP. subcordiformis, and will improve fundamental research and enlarge application of this alga.

关 键 词：bar glass-bead agitation nuclear transformation particle bombardment Platymonas subcordiformis 

分 类 号：Q943[生物学—植物学]